TSG101 Antibody - #DF8427

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产品: TSG101 抗体
货号: DF8427
描述: Rabbit polyclonal antibody to TSG101
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 45 kDa; 44kD(Calculated).
蛋白号: Q99816
RRID: AB_2841675

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
TSG101 Antibody detects endogenous levels of total TSG101.
RRID:
AB_2841675
引用格式: Affinity Biosciences Cat# DF8427, RRID:AB_2841675.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ESCRT I complex subunit TSG101; ESCRT-I complex subunit TSG101; TS101_HUMAN; TSG 10; TSG 101; TSG10; Tsg101; Tumor susceptibility gene 10; Tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; Tumor susceptibility protein; Tumor susceptibility protein isoform 3; VPS 23; VPS23;

抗原和靶标

免疫原:
Uniprot:

Q99816(TS101_HUMAN) >>Visit HPA database.

基因/基因ID:
TSG101(7251)
表达:
Q99816 TS101_HUMAN:

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

序列:
MAVSESQLKKMVSKYKYRDLTVRETVNVITLYKDLKPVLDSYVFNDGSSRELMNLTGTIPVPYRGNTYNIPICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHEWKHPQSDLLGLIQVMIVVFGDEPPVFSRPISASYPPYQATGPPNTSYMPGMPGGISPYPSGYPPNPSGYPGCPYPPGGPYPATTSSQYPSQPPVTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
91
Sheep
91
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99816 作为底物

Site PTM Type Enzyme
A2 Acetylation
S6 Phosphorylation
K14 Acetylation
Y15 Phosphorylation
K16 Acetylation
Y32 Phosphorylation
S48 Phosphorylation
T56 Phosphorylation
Y63 Phosphorylation
K90 Acetylation
K101 Ubiquitination
T220 Phosphorylation
S222 Phosphorylation
T225 Phosphorylation
K237 Acetylation
K237 Ubiquitination
K257 Ubiquitination
K269 Ubiquitination
K286 Ubiquitination
K292 Ubiquitination
K293 Ubiquitination
S299 Phosphorylation
S300 Phosphorylation
K304 Ubiquitination
S309 Phosphorylation
K361 Ubiquitination
K379 Ubiquitination
Y390 Phosphorylation

研究背景

功能:

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan. It may also play a role in the extracellular release of microvesicles that differ from the exosomes.

翻译修饰:

Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.

细胞定位:

Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side. Late endosome membrane>Peripheral membrane protein. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Midbody>Midbody ring. Nucleus.
Note: Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Nuclear localization is cell cycle-dependent. Interaction with CEP55 is required for localization to the midbody during cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

亚基结构:

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry. Interacts with VPS37A, VPS37B and VPS37C. Interacts with DMAP1. Interacts with ubiquitin. Interacts with stathmin, GMCL and AATF (By similarity). Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry. Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex. Interacts with GGA1 and GGA3. Interacts (via UEV domain) with PDCD6IP/AIP1. Interacts with VPS28, SNF8 and VPS36. Self-associates. Interacts with MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis but not for viral budding. Interacts with PDCD6. Interacts with LITAF. Interacts with MGRN1. Interacts with ARRDC1; recruits TSG101 to the plasma membrane.

(Microbial infection) Interacts with HIV-1 p6.

(Microbial infection) Interacts with human spumavirus Gag.

(Microbial infection) Interacts with HTLV-1 Gag.

(Microbial infection) Interacts with Ebola virus VP40.

(Microbial infection) Interacts with EIAV p9; the interaction has been shown in vitro.

(Microbial infection) Interacts with Lassa virus protein Z.

(Microbial infection) Interacts with hepatitis E virus protein ORF3.

蛋白家族:

The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.

The coiled coil domain may interact with stathmin.

The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.

Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

文献引用

1). Isothiazolinone dysregulates the pattern of miRNA secretion: Endocrine implications for neurogenesis. Environment international (PubMed: 37939439) [IF=11.8]
2). Mesenchymal Stem Cell Derived Exosomes as Nanodrug Carrier of Doxorubicin for Targeted Osteosarcoma Therapy via SDF1-CXCR4 Axis. International Journal of Nanomedicine (PubMed: 35959282) [IF=8.0]

Application: WB    Species: Mouse    Sample: BM-MSCs cells

Figure 1 Characterization of exosomes: the size distributions of blank exosome (A) and exosome-doxorubicin (B) measured by NTA. The mean particle diameters were 141.6 nm for free exosome and 178.1 nm for exosome-doxorubicin. The morphology of blank exosome (C) and exosome-doxorubicin (D) as observed by TEM. (E) Western blotting analysis of the exosomal proteins CD81 and TSG101.
3). Selective CDK9 knockdown sensitizes TRAIL response by suppression of antiapoptotic factors and NF-kappaB pathway. Apoptosis (PubMed: 37060507) [IF=7.2]
4). Discovery and validation of extracellular vesicle‐associated miRNAs as noninvasive detection biomarkers for early‐stage non‐small‐cell lung cancer. Molecular Oncology (PubMed: 33340250) [IF=6.6]

Application: WB    Species: Human    Sample: lung tissues

Figure 1. Characterisation of EVs derived from the serum and plasma of NSCLC patients and controls. (a) The shape and structure of serum and plasma EVs isolated by EXOquick kit under TEM. The red arrow represents EVs with typical characteristics (scale bars are 200 nm). (b) The size of EVs derived from control groups and NSCLC groups was analysed by NTA. (c) Western blots of EVs membrane markers, including Alix, CD63, TSG101, CD9, and one negative marker ALB.
5). Mammary Epithelial Cell-Derived Exosomal miR-221-3p Regulates Macrophage Polarization by Targeting Igf2bp2 during Mastitis. Journal of Agricultural and Food Chemistry (PubMed: 37757458) [IF=6.1]
6). Exosomes derived from smooth muscle cells ameliorate diabetes‐induced erectile dysfunction by inhibiting fibrosis and modulating the NO/cGMP pathway. Journal of Cellular and Molecular Medicine (PubMed: 33009701) [IF=5.3]

Application: WB    Species: Rat    Sample: CCSMCs

FIGURE 1Cell identification and exosome characterization. (A) Representative flow cytometry histograms of BMSCs and ADSCs show positive staining for CD29 and CD90 but not for CD34 and CD45. (B) BMSCs and ADSCs were successfully induced into osteoblasts (positively stained with Alizarin Red S) and adipocytes (positively stained with Oil Red O). The magnification is 100×. (C) Representative immunofluorescence results of CCSMCs show positive expression for α-SMA and desmin. Scale bars = 50 μm. (D) Exosomes derived from CCSMCs, BMSCs and ADSCs were observed using transmission electron microscopy, and the particle size distributions of the exosomes were measured by nanoparticle tracking analysis. Scale bars = 100 nm. (E) Representative results of Western blot analysis of exosomes derived from CCSMCs, BMSCs and ADSCs show positive expression for CD9, CD63 and TSG101 but not for calnexin. CCSMC: corpus cavernosum smooth muscle cell; BMSC: bone marrow stem cell; ADSC: adipose-derived stem cell; CCSMC-EXOs: exosomes derived from corpus cavernosum smooth muscle cells; BMSC-EXOs: exosomes derived from bone marrow stem cells; ADSC-EXOs: exosomes derived from adipose-derived stem cells; α-SMA: α-smooth muscle actin; DAPI: 4’,6-diamidino-2-phenylindole
7). Pulmonary Delivery of Extracellular Vesicle-Encapsulated Dinaciclib as an Effective Lung Cancer Therapy. Cancers (PubMed: 35884614) [IF=5.2]
8). Expression level and diagnostic value of exosomal NEAT1/miR‐204/MMP‐9 in acute ST‐segment elevation myocardial infarction. IUBMB Life (PubMed: 32916037) [IF=4.6]

Application: WB    Species: human    Sample: serum exosomes

FIGURE 2 |Exosome identification. (a) Structure of exosomes under TEM, magnification 150,000×. (b) Western blot showed the expression of exosome markers CD63 and TSG101
9). Human umbilical cord mesenchymal stem cell-derived exosomes promote neurological function recovery in rat after traumatic brain injury by inhibiting the activation of microglia and astrocyte. Regenerative Therapy (PubMed: 36092501) [IF=4.3]
10). Exosomal connexin 43 regulates the resistance of glioma cells to temozolomide. Oncology Reports (PubMed: 33649836) [IF=4.2]

Application: WB    Species: Human    Sample: U251s and U251r cells

Figure 2. Isolation and identification of exosomes from U251s and U251r cells. (A) Exosomes were isolated from U251s or U251r cells by differential velocity centrifugation, and representative images of sExo and rExo were captured under a transmission electron microscope. Scale bar, 200 nm. (B) sExo and rExo diameters were measured using the Zetasizer Nano ZS90 particle size analyzer. The size distribution of the vesicles was concentrated in the range of 40–120 nm. (C) Western blotting was used to examine the expression of exosomal markers CD63 and TSG101 in U251s cells, sExo and rExo; GAPDH was used as an endogenous reference. (D and E) Quantitative analysis of the protein expression level of CD63 and TSG101. **P<0.01, ***P<0.001. U251r, temozolomide-resistant U251 glioma cells; U251s, temozolomide-sensitive U251 glioma cells; sExo, exosomes of U251s cells; rExo, exosomes of U251r cells.
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