ABCG5 Antibody - #DF8401

Figure 4. The effects of BPSIP, BPS, or E2 exposure on the regulation of hepatic cholesterol biosynthesis and intestinal absorption in offspring mice. Liver qPCR analysis of selected cholesterol biosynthesis key genes in (A) females and (B) males (𝑁=24, 𝑛=6 per group/sex). Immunoblotting of liver HMGCR in (C) females and (D) males (𝑁=28, 𝑛=7/group for female; 𝑁=32, 𝑛=8/group for male). (E) Immunofluorescence staining of HMGCR in livers of female offspring mice (the scale bar=50μ⁢m). (F) Quantification for HMGCR per filed in females (𝑁=12, 𝑛=6 per group). (G) Immunofluorescence staining of HMGCR in livers of male offspring mice (the scale bar=50μ⁢m). (H) Quantification for HMGCR per filed in males (𝑁=12, 𝑛=6 per group). Intestinal qPCR of Npc1l1 and Mttp in (I) females and (J) males (𝑁=32, 𝑛=8 per group/sex). Immunoblotting of intestinal NPC1L1 in (K) females and (L) males (𝑁=28, 𝑛=7 per group/sex). All data were provided as means±SEM. Statistical analysis was performed using Student’s 𝑡-test (F,H) and one-way ANOVA followed by post hoc Tukey’s test (A–D,I–L). *𝑝

Figure 1The effect of clinical ketosis on the abundance of key molecules in cholesterol synthesis, transport, and excretion. Liver tissue from healthy cows and cows with clinical ketosis was collected and digested for analysis of mRNA and protein abundance. (A) Relative mRNA abundance of SREBF2, HMGCR, ACAT2, ABCA1, ABCG5, and ABCG8; (B) representative western blots; and (C) relative protein abundance of SREBF2, HMGCR, ACAT2, ABCA1, and ABCG5. Comparisons among groups were calculated using a 2-tailed unpaired Student's t-test.