产品: | PRAS40 抗体 |
货号: | DF8396 |
描述: | Rabbit polyclonal antibody to PRAS40 |
应用: | WB IF/ICC |
反应: | Human |
预测: | Pig, Bovine, Horse, Sheep, Dog |
分子量: | 27 kDa; 27kD(Calculated). |
蛋白号: | Q96B36 |
RRID: | AB_2841651 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8396, RRID:AB_2841651.
展开/折叠
40 kDa proline rich AKT substrate; 40 kDa proline-rich AKT substrate; AKT1 S1; AKT1 substrate 1 (proline rich); AKT1 substrate 1; AKT1S 1; AKT1S1; AKTS1_HUMAN; Lobe; MGC2865; PRAS 40; PRAS; PRAS40; Proline rich akt substrate; Proline rich Akt substrate 40 kDa; Proline-rich AKT1 substrate 1;
抗原和靶标
Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
- Q96B36 AKTS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96B36 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T32 | Phosphorylation | Uniprot | |
R40 | Methylation | Uniprot | |
R51 | Methylation | Uniprot | |
T73 | Phosphorylation | Uniprot | |
S88 | Phosphorylation | Uniprot | |
T90 | Phosphorylation | Uniprot | |
S92 | Phosphorylation | Uniprot | |
T97 | Phosphorylation | Uniprot | |
S183 | Phosphorylation | P42345 (MTOR) | Uniprot |
T198 | Phosphorylation | Uniprot | |
S202 | Phosphorylation | Uniprot | |
S203 | Phosphorylation | Uniprot | |
S211 | Phosphorylation | Uniprot | |
S212 | Phosphorylation | Uniprot | |
S221 | Phosphorylation | P42345 (MTOR) | Uniprot |
T246 | Phosphorylation | P11309 (PIM1) , P31749 (AKT1) , P31751 (AKT2) , O43781 (DYRK3) | Uniprot |
S247 | Phosphorylation | Uniprot | |
K251 | Methylation | Uniprot | |
K251 | Ubiquitination | Uniprot | |
Y256 | Phosphorylation | Uniprot |
研究背景
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Phosphorylated by AKT1. Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1.
Cytoplasm>Cytosol.
Note: Found in the cytosolic fraction of the brain.
Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Interacts directly with RPTOR. The phosphorylated form interacts with 14-3-3 proteins.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)
文献引用
Application: WB Species: Human Sample:
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