Phospho-Chk1 (Ser317) Antibody - #AF3007

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产品: 磷酸化 Chk1 (Ser317) 抗体
货号: AF3007
描述: Rabbit polyclonal antibody to Phospho-Chk1 (Ser317)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: O14757
RRID: AB_2834446

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 50ul RMB¥ 1300 现货
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Chk1 (Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 317.
RRID:
AB_2834446
引用格式: Affinity Biosciences Cat# AF3007, RRID:AB_2834446.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C85740; Cell cycle checkpoint kinase; Checkpoint , S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog (S. pombe); CHEK 1; Chek1; Chk 1; Chk1; CHK1 checkpoint homolog (S. pombe); CHK1_HUMAN; EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae);

抗原和靶标

免疫原:

A synthesized peptide derived from human Chk1 around the phosphorylation site of Ser317.

基因/基因ID:
CHEK1(1111)
描述:
DNA damage induced protein phosphorylation; regulation of mitotic centrosome separation; regulation of S phase; peptidyl-threonine phosphorylation; DNA repair; chromatin-mediated maintenance of transcription; negative regulation of mitosis;

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

文献引用

1). Sterigmatocystin-induced checkpoint adaptation depends on Chk1 in immortalized human gastric epithelial cells in vitro. ARCHIVES OF TOXICOLOGY, 2017 (PubMed: 26914363) [IF=4.8]

Application: WB    Species: human    Sample: human gastric epithelial cells

Fig. 4 Chk1 activation plays an important role in the ST-induced G2 arrest. a Western blot results showed the effects of different concentrations of ST treatment on the phosphorylation of Chk1 (Ser-280), (Ser-345) and (Ser-317), as well as the expression of Chk1 in GES-1 cells.
2). Temporal analysis of lung injury induced by real-ambient PM2 .5 exposure in mice. Environmental toxicology, 2024 (PubMed: 37782690) [IF=4.4]

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