TLS/FUS Antibody - #DF8391

Fig. 3: The upregulated expression of miR-18b in exosomes enhanced migration and invasion of breast cancer cells. A qRT-PCR detected miR-18b expression in cancer cells co-cultured with NFs and CAFs-derived exosomes (n = 3). B, C Transwell experiments demonstrated the effect of miR-18b on the ability of breast cancer cells in migration and invasion (n = 3). Scale bar, 50 μm. D RBPDB analysis was used to predict the specific interaction between the miR-18b sequence and RBP motifs (threshold 0.7). E Using real-time PCR to measure the expression of miR-18b in CAFs-derived exosomes transfected with specific siRNA targeting FUS, ACO1, or YTHDC1 (n = 3). F Real-time PCR analysis detected the expression of miR-18b after silencing FUS in CAFs (n = 3). G The miRNA pull-down experiment analyzed the interaction of miR-18b and miR-18b mutant with FUS in the cytoplasm, nucleus, and exosomal lysates of CAFs, biotinylated poly. H As a negative control (n = 3). I Anti-FUS antibody (or IgG as a control) was used for RIP detection of CAFs cells or exosomal lysates (n = 3). * indicates p