MUC2 Antibody - #DF8390

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产品: MUC2 抗体
货号: DF8390
描述: Rabbit polyclonal antibody to MUC2
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Rabbit
蛋白号: Q02817
RRID: AB_2827875

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
MUC2 Antibody detects endogenous levels of total MUC2.
RRID:
AB_2827875
引用格式: Affinity Biosciences Cat# DF8390, RRID:AB_2827875.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Intestinal mucin 2; Intestinal mucin-2; MLP; MUC 2; MUC-2; Muc2; MUC2_HUMAN; Mucin 2; Mucin 2 intestinal; Mucin 2 intestinal/tracheal; Mucin 2 oligomeric mucus/gel forming; Mucin 2 precursor; Mucin 2 tracheal; Mucin like protein; Mucin-2; Mucin2; SMUC;

抗原和靶标

免疫原:

A synthesized peptide derived from human MUC2, corresponding to a region within N-terminal amino acids.

文献引用

1). A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon. Acta Biomaterialia, 2022 (PubMed: 35245681) [IF=9.4]
2). Fourteen composite probiotics alleviate type 2 diabetes through modulating gut microbiota and modifying M1/M2 phenotype macrophage in db/ db mice. PHARMACOLOGICAL RESEARCH, 2020 (PubMed: 32818655) [IF=9.1]

Application: IHC    Species: mouse    Sample: colon

Fig. 4.| Effects of the composite probiotics on intestinal barrier function.The protein expression of claudin-1 (G), occludin-1 (H), ZO-1 (I) and mucin-2 (J)determined by immunohistochemistry (original magnification 200×) (n = 4 images/group).
3). Tremella fuciformis polysaccharides ameliorated ulcerative colitis via inhibiting inflammation and enhancing intestinal epithelial barrier function. International Journal of Biological Macromolecules, 2021 (PubMed: 33744251) [IF=7.7]

Application: IF/ICC    Species: human    Sample: Caco-2 cells

Fig. 7.| Effect of Tremella fuciformis polysaccharides on regulating the expressions of proteins for intestinal barrier and mucus barrier in LPS-stimulated Caco-2 cells. s. (A) Representative fluorescent images of ZO-1 in Caco-2 cells. (B) The fluorescence intensity of ZO-1 in Caco-2 cells. (C) Representative fluorescent images of occludin in Caco-2 cells. (D) The fluorescence intensity of occludin in Caco-2 cells. (E) Representative fluorescent images of Muc-2 in Caco-2 cells.
4). Feedback delivery of BMP 7 on the pathological oxidative stress via smart hyaluronic acid hydrogel potentiated the repairing of the gut epithelial integrity. International journal of biological macromolecules, 2024 (PubMed: 39447783) [IF=7.7]
5). Commensal cow Roseburia reduces gut-dysbiosis-induced mastitis through inhibiting bacterial translocation by producing butyrate in mice. Cell Reports, 2022 (PubMed: 36417859) [IF=7.5]

Application: IHC    Species: Mouse    Sample:

Figure 2FMT from cows with mastitis to mice induces mastitis and disrupts the blood-milk and gut barrier in mice
6). Composite probiotics alleviate type 2 diabetes by regulating intestinal microbiota and inducing GLP-1 secretion in db/db mice. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 32035395) [IF=6.9]

Application: IHC    Species: Mice    Sample: colon tissue

Fig. 6. The composite probiotics improved intestinal mucosal barrier function. (A, B) The colon histology in HE staining (original magnification 100 × and 200 ×) (n = 4 images/group). (C, D) The claudin-1 (original magnification 200 ×) and mucin-2 (original magnification 400 ×) protein expression of the colon tissue in immunohistochemistry staining (n = 4 images/group). (E, F) The mRNA levels of claudin-1 and mucin-2 in the colon tissue were determined by qRT-PCR (n = 5). (G, H) The levels of LPS in the faeces and serum were determined by ELISA kits (n = 6). Mean integral optical density values of claudin-1 and mucin-2 were analyzed by Image-Pro Plus 6.0. Data are presented as mean ± SD values. ## P < 0.01 vs normal group; * P < 0.05, ** P < 0.01 vs model group.
7). Yiqi Huayu Jiedu formula inhibits JAK2/STAT3-mediated partial EMT in treating chronic atrophic gastritis. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2025 (PubMed: 39799895) [IF=6.7]

Application: IF/ICC    Species: human    Sample: GES-1 cells

Fig. 4. CDCA induced precancerous gastric disease phenotype in GES-1 cells. (A) Morphological changes in cell morphology of GES-1 cells treated with CDCA at different concentrations. (B) Protein expression levels of CDX2, KLF4, Villin in GES-1 cells treated with CDCA. (C) Cellular Immunofluorescent assay were performed on GES-1 cells treated with 400 µM CDCA for 24 h to assess the expression and localization of MUC2 and Villin.
8). Oat Peptides Alleviate Dextran Sulfate Sodium Salt-Induced Colitis by Maintaining the Intestinal Barrier and Modulating the Keap1-Nrf2 Axis. Nutrients, 2023 (PubMed: 38140314) [IF=5.9]

Application: IHC    Species: Mouse    Sample: colon tissue

Figure 3 OPs repair the intestinal mechanical barrier. (A) HE staining results of colon tissue; (B) AB staining results; (C) PAS staining results; (D) IHC results for Muc2 protein; (E) mRNA expression of ZO-1, occludin and claudin 1; (F) Western blotting results for ZO-1, occludin and claudin 1; (G) normalized analysis results for the ZO-1, occludin and claudin 1 proteins. Data are presented as mean ± SD (A–D,G) (n = 3–5 per group).
9). Fucoxanthin Alleviates Dextran Sulfate Sodium-Induced Colitis and Gut Microbiota Dysbiosis in Mice. Journal of agricultural and food chemistry, 2024 (PubMed: 38355398) [IF=5.7]
10). Intestinal changes in permeability, tight junction and mucin synthesis in a mouse model of Alzheimer's disease. International journal of molecular medicine, 2023 (PubMed: 37830152) [IF=5.7]

Application: WB    Species: Mouse    Sample: intestinal tissue

Figure 6 Variation in number of GCs and MUC2 expression in intestinal mucosa. (Aa-h) Alcian blue-Periodic acid Schiff staining showed GCs and (Ba-h) immunohistochemistry showed MUC2 in the ileum and proximal colon of WT and dTg mice at 6 and 12 months. (C) Number of GCs, as determined by histochemical staining of the ileum and proximal colon. (D) MUC2 expression area in the ileum and proximal colon was determined by immunohistochemistry (n=6/group). Scale bar, 50 µm. (E) Protein levels of MUC2 in extracts of intestinal tissue were detected by western blotting. (F) Quantification of the western blot results (n=4/group). *P

Application: IHC    Species: Mouse    Sample: intestinal tissue

Figure 6 Variation in number of GCs and MUC2 expression in intestinal mucosa. (Aa-h) Alcian blue-Periodic acid Schiff staining showed GCs and (Ba-h) immunohistochemistry showed MUC2 in the ileum and proximal colon of WT and dTg mice at 6 and 12 months. (C) Number of GCs, as determined by histochemical staining of the ileum and proximal colon. (D) MUC2 expression area in the ileum and proximal colon was determined by immunohistochemistry (n=6/group). Scale bar, 50 µm. (E) Protein levels of MUC2 in extracts of intestinal tissue were detected by western blotting. (F) Quantification of the western blot results (n=4/group). *P

Application: IF/ICC    Species: Mouse    Sample: intestinal tissue

Figure 7 Variation in MUC2 and WGA in intestinal mucosa. (Aa-p) IF showed MUC2 and WGA in ileum of WT and dTg mice at 6 and 12 months. (B) MUC2- and (C) WGA-positive area was determined by IF in ileum (n=6/group). IF showed MUC2 and WGA in (Da-p) proximal colon of WT and dTg mice at 6 and 12 months. (E) MUC2- and (F) WGA-positive area was determined by IF in proximal colon (n=6/group). *P
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