Phospho-IRE1 (Ser724) Antibody - #DF8322

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产品: 磷酸化 IRE1 (Ser724) 抗体
货号: DF8322
描述: Rabbit polyclonal antibody to Phospho-IRE1 (Ser724)
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: O75460
RRID: AB_2841598

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 50ul RMB¥ 1800 现货
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 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-IRE1 (Ser724) Antibody detects endogenous levels of IRE1 only when phosphorylated at Ser724.
RRID:
AB_2841598
引用格式: Affinity Biosciences Cat# DF8322, RRID:AB_2841598.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; Ern1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1;

抗原和靶标

免疫原:

Synthetic peptide corresponding to Human IRE1 around the phosphorylation site of Ser724.

基因/基因ID:
ERN1(2081)

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

文献引用

1). TRAPPC1 is essential for the maintenance and differentiation of common myeloid progenitors in mice. EMBO reports, 2023 (PubMed: 36440617) [IF=6.5]
2). Acute exposure to polystyrene nanoplastics induces unfolded protein response and global protein ubiquitination in lungs of mice. Ecotoxicology and environmental safety, 2024 (PubMed: 38865938) [IF=6.2]
3). Polystyrene nanoplastics promote CHIP-mediated degradation of tight junction proteins by activating IRE1α/XBP1s pathway in mouse Sertoli cells. Ecotoxicology and Environmental Safety, 2022 (PubMed: 36446169) [IF=6.2]

Application: WB    Species: Mouse    Sample: TM4 cells

Fig. 4. PS-NPs activated UPR in TM4 Sertoli cells. (a) TM4 cells were treated with indicated concentrations of PS-NPs for 24 h. The protein levels of p-IRE1α, GRP78, ATF6α, and β-actin were measured using the Western blot analysis. Results were presented as the mean ± SD (n = 3). *p 
4). Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. Frontiers in Pharmacology, 2019 (PubMed: 31632274) [IF=5.6]

Application: WB    Species: rat    Sample: liver

FIGURE 4 | PA treatment attenuated HFD-induced ER stress in rats. (A) Representative immunoreactive bands of GRP78, PERK, p-PERK, IRE1α, p-IRE1α, and ATF6
5). Dl-3-n-Butylphthalide Ameliorates Diabetic Nephropathy by Ameliorating Excessive Fibrosis and Podocyte Apoptosis. Frontiers in Pharmacology, 2021 (PubMed: 34497508) [IF=5.6]

Application: WB    Species: Mice    Sample: kidney tissues

FIGURE 6 DL-NBP treatment inhibits diabetes-induced elevated ERS in kidney. (A–F) Western blotting and quantification of ERS-elevated protein expression [p-PERK (A,B), p-IRE1α (A,C), GRP78 (A,D), ATF-6 (A,E), CHOP (A,F)] in kidney of mice from each group (n = 3). Protein expression was normalized to the expression of β-actin. (G,H) Representative images of immunohistochemical staining of CHOP in kidney of mice from each group (n = 3). Scale bar = 20 μm. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001 vs. db/m control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. db/db mice.
6). Exosomes from adipose-derived mesenchymal stem cells can attenuate liver injury caused by minimally invasive hemihepatectomy combined with ischemia-reperfusion in minipigs by modulating the endoplasmic reticulum stress response. Life sciences, 2023 (PubMed: 36966916) [IF=5.2]
7). Icariin improves the cognitive function of APP/PS1 mice via suppressing endoplasmic reticulum stress. LIFE SCIENCES, 2019 (PubMed: 31400352) [IF=5.2]

Application: WB    Species: mouse    Sample: hippocampus

Fig.5| Effects of ICA on ER stress in the hippocampus of APP/PS1 mice. ER stress was activated in the hippocampus of APP/PS1 mice evidenced by the high levels of GRP78, ATF6, and p-IRE1α. ICA treatment decreased GRP78 level, not affected the ATF6, IRE1α and IRE1αphosphorylation. (A) Immunoblots.
8). Modulation of Ire1-Xbp1 Defense Pathway in Encephalomyocarditis Virus-Infected HeLa Cells. Viruses, 2025 (PubMed: 40143290) [IF=4.7]
9). The endocrine disruptor vinclozolin causes endothelial injury via eNOS/Nox4/IRE1α signaling. European journal of pharmacology, 2024 (PubMed: 38901528) [IF=4.2]
10). Modulation of non‐steroidal anti‐inflammatory drug‐induced, ER stress‐mediated apoptosis in Caco‐2 cells by different polyphenolic antioxidants: a mechanistic study. Journal of Pharmacy and Pharmacology, 2020 (PubMed: 32716561) [IF=2.8]

Application: WB    Species: human    Sample: Caco-2 cells

Figure 7| Expression of IRE-1a/JNK1/2 proteins and their phosphorylated forms (a) in Caco-2 cells after treatment with ER stressors (INDO, DIC,TUN, TGN) in the presence of the test compounds (EGCG, HYPO, PHY, ML385) for 48 h.
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