Phospho-SGK1 (Ser422) Antibody - #AF3001

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产品: 磷酸化 SGK1 (Ser422) 抗体
货号: AF3001
描述: Rabbit polyclonal antibody to Phospho-SGK1 (Ser422)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: O00141
RRID: AB_2834440

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 200ul RMB¥ 3200 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-SGK1 (Ser422) Antibody detects endogenous levels of SGK1 only when phosphorylated at Serine 422.
RRID:
AB_2834440
引用格式: Affinity Biosciences Cat# AF3001, RRID:AB_2834440.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

OTTHUMP00000017247; Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serine/threonine-protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase 1; Serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK 1; SGK; SGK1; Sgk1 variant i3; SGK1_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human SGK1 around the phosphorylation site of Ser422.

基因/基因ID:
SGK1(6446)
描述:
This gene encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis.

研究领域

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

文献引用

1). The regulation of MFG-E8 on the mitophagy in diabetic sarcopenia via the HSPA1L-Parkin pathway and the effect of D-pinitol. Journal of cachexia, sarcopenia and muscle, 2024 (PubMed: 38553831) [IF=9.4]

Application: WB    Species: Mouse    Sample:

Figure 6 WB strips and quantitative analysis of SGK, FOXO1, and STAT3. The diagram of the signalling pathway. (A) WB strips of SGK, FOXO1, and STAT3 in part 3 protocol, and quantitative analysis (*P < 0.05; **P < 0.01; ***P < 0.001). (B) The diagram of the signalling pathway related to MFG-E8 and mitophagy.
2). Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12. International journal of biological sciences, 2024 (PubMed: 38904021) [IF=8.2]
3). Eplerenone inhibits UUO-induced lymphangiogenesis and cardiac fibrosis by attenuating inflammatory injury. International Immunopharmacology, 2022 (PubMed: 35428023) [IF=4.8]
4). Eplerenone ameliorates lung fibrosis in unilateral ureteral obstruction rats by inhibiting lymphangiogenesis. Experimental and Therapeutic Medicine, 2022 (PubMed: 36160894) [IF=2.4]

Application: WB    Species: Rat    Sample: lung tissue

Figure 4 Effect of UUO and EPL on expression of downstream molecules of MRs in lung tissue. Immunohistochemical staining for (A) SGK-1 and (B) NF-κB. Magnification, x400. (C) Protein expression of p-SGK-1, SGK-1, MR and NF-κB as detected by western blotting. (D) SGK-1 and (E) NF-κB positive expression. (F) p-SGK-1 and (G) SGK-1 protein expression in each group. (H) Ratio of p-SGK-1 to SGK-1. (I) MR and (J) NF-κB protein expression. mRNA expression of (K) SGK-1 and (L) NF-κB as detected by RT-qPCR. Data are presented as the mean ± standard deviation (n=6). #P

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