产品: CD53 抗体
货号: DF8263
描述: Rabbit polyclonal antibody to CD53
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 24 kDa; 24kD(Calculated).
蛋白号: P19397
RRID: AB_2841552

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Bovine(100%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)
克隆:
Polyclonal
特异性:
CD53 Antibody detects endogenous levels of total CD53.
RRID:
AB_2841552
引用格式: Affinity Biosciences Cat# DF8263, RRID:AB_2841552.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AI323659; Antigen MOX44 identified by monoclonal antibody MRC-OX44; CD 53; CD53; CD53 antigen; CD53 antigen, isoform CRA_b; CD53 glycoprotein; Cd53 molecule; CD53 tetraspan antigen; CD53_HUMAN; Cell surface glycoprotein CD53; Leukocyte antigen MRC OX-44; Leukocyte surface antigen CD53; MOX 44; MOX44; OTTHUMP00000059505; Ox-44; OX44; RATOX44; Tetraspanin 25; Tetraspanin-25; Tetraspanin25; Transmembrane glycoprotein; TSPAN 25; Tspan-25; TSPAN25;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P19397 CD53_HUMAN:

B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.

序列:
MGMSSLKLLKYVLFFFNLLFWICGCCILGFGIYLLIHNNFGVLFHNLPSLTLGNVFVIVGSIIMVVAFLGCMGSIKENKCLLMSFFILLLIILLAEVTLAILLFVYEQKLNEYVAKGLTDSIHRYHSDNSTKAAWDSIQSFLQCCGINGTSDWTSGPPASCPSDRKVEGCYAKARLWFHSNFLYIGIITICVCVIEVLGMSFALTLNCQIDKTSQTIGL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity).

细胞定位:

Cell membrane. Cell junction. Membrane>Multi-pass membrane protein.
Note: Concentrates in localized microdomains along the plasma membrane at the contact sites between cells of fused myotubes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.

亚基结构:

Interacts with SCIMP.

蛋白家族:

Belongs to the tetraspanin (TM4SF) family.

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