Phospho-JAK2 (Tyr1007) Antibody - #AF3022

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产品: 磷酸化 JAK2 (Tyr1007) 抗体
货号: AF3022
描述: Rabbit polyclonal antibody to Phospho-JAK2 (Tyr1007)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: O60674
RRID: AB_2834429

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产品描述

来源:
Rabbit
应用:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-JAK2 (Tyr1007) Antibody detects endogenous levels of JAK2 only when phosphorylated at Tyrosine 1007.
RRID:
AB_2834429
引用格式: Affinity Biosciences Cat# AF3022, RRID:AB_2834429.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

JAK 2; JAK-2; JAK2; JAK2_HUMAN; Janus Activating Kinase 2; Janus kinase 2 (a protein tyrosine kinase); Janus kinase 2; JTK 10; JTK10; kinase Jak2; OTTHUMP00000043260; THCYT3; Tyrosine protein kinase JAK2; Tyrosine-protein kinase JAK2;

抗原和靶标

免疫原:

A synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr1007.

基因/基因ID:
JAK2(3717)
描述:
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Targeted pharmacokinetics and bioinformatics screening strategy reveals JAK2 as the main target for Xin-Ji-Er-Kang in treatment of MIR injury. Biomedicine & Pharmacotherapy, 2022 (PubMed: 36271569) [IF=6.9]

Application: WB    Species: Human    Sample:

Fig. 5. Effects of XJEK on JAK2/STAT1/STAT3 pathway and myocardial cell apoptosis after MIR injury. A Representative figure of JAK2, pJAK2, STAT3, pSTAT3, STAT1, pSTAT1 protein. B-E Quantitative analyses of JAK2, pJAK2, STAT3, pSTAT3, STAT1, pSTAT1 protein (n = 6). F Representative photographs of TUNEL/DAPI double staining of the cardiomyocytes after MIR (×630). H The quantitative analysis of TUNEL/DAPI (n = 6). G Representative figures of expression of Bax and Bcl-2. I Quantitative analyses of Bax and Bcl-2 protein (n = 6). *P 
2). Puerarin suppresses macrophage M1 polarization to alleviate renal inflammatory injury through antagonizing TLR4/MyD88-mediated NF-κB p65 and JNK/FoxO1 activation. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38905846) [IF=6.7]
3). Schisandra chinensis Lignans Exert Antidepressant Effects by Promoting BV2 Microglia Polarization toward the M2 Phenotype through the Activation of the Cannabinoid Receptor Type-2–Signal Transducer and Activator of Transcription 6 Pathway. Journal of Agricultural and Food Chemistry, 2022 (PubMed: 36349542) [IF=5.7]
4). A novel small molecule RK-019 inhibits FGFR2-amplification gastric cancer cell proliferation and induces apoptosis in vitro and in vivo. Frontiers in Pharmacology, 2022 (PubMed: 36210834) [IF=5.6]
5). Palmitic Acid Inhibits the Growth and Metastasis of Gastric Cancer by Blocking the STAT3 Signaling Pathway. Cancers, 2023 (PubMed: 36672337) [IF=5.2]
6). Oxalate stimulates macrophage secretion of prostaglandin E2 to promote renal tubular epithelial cell osteogenesis. Life sciences, 2025 (PubMed: 39986650) [IF=5.2]
7). Maresin 1 alleviates sevoflurane-induced neuroinflammation in neonatal rats via JAK2/STAT3/IL-6 pathways. International Immunopharmacology, 2022 (PubMed: 35729840) [IF=4.8]

Application: WB    Species: Rat    Sample: hippocampus

Fig. 5. MaR1 inhibits the activation of JAK2/STAT3 signaling pathway. A-C MaR1 deactivated JAK2/STAT3 pathway in hippocampus shown by Western blotting using JAK2/STAT3 antibodies (A) and quantitative analysis (B, C). One-way ANOVA, [pJAK2] F(3, 8) = 6.753, p = 0.0154; [pSTAT3] F(3, 8) = 5.325, p = 0.0245, n = 3. D,E MaR1 inactivated GSK3β pathway in hippocampus shown by Western blotting (D) and quantitative analysis (E). One-way ANOVA, [pS9] F(3, 8) = 7.565, p = 0.0112, n = 3. F Predicted STAT3′ s binding motifs on the promoter of IL-6 were examined by dual luciferase reporter assay. Unpaired t-test, [Motif3] t = 3.546 df = 4, p = 0.0116, n = 3. * p < 0.05; # p < 0.05. Data were presented as mean ± SEM.
8). Tert-Butylhydroquinone Mitigates T-2-Toxin-Induced Testicular Dysfunction by Targeting Oxidative Stress, Inflammation, and Apoptosis in Rats. Toxics, 2024 (PubMed: 38787114) [IF=4.6]

Application: WB    Species: Rat    Sample:

Figure 3 tBHQ alleviates the inflammatory response induced by T-2. (A) mRNA expression levels of inflammatory factors; (B,D) protein expression levels and phosphorylation of JAK, represented by grayscale values; (C,E) protein expression levels and phosphorylation of STAT3, represented by grayscale values. n = 8. * represents a significant difference, with p < 0.05. ** represents a significant difference, with p < 0.01.
9). Transcriptional Regulation of Voltage-Gated Sodium Channels Contributes to GM-CSF-Induced Pain. JOURNAL OF NEUROSCIENCE, 2019 (PubMed: 31015342) [IF=4.4]

Application: WB    Species: rat    Sample: DRG neurons

Figure 5. | GM-CSF increase the mRNA expression level of Nav1.7, Nav1.8, 822 Nav1.9 channel through Jak2-Stat3 signaling pathway. (A) Relative expression of 823 p-Jak1, p-Jak2, p-Jak3, p-stat3 and p-stat5 in DRG neurons after incubation with 824 GM-CSF for 25 mins. (n=3, unpaired t-test, *P < 0.05 as compared to control)
10). Hyperuricemia induces lipid disturbances mediated by LPCAT3 upregulation in the liver. FASEB JOURNAL, 2020 (PubMed: 32780898) [IF=4.4]

Application: WB    Species: human    Sample: L02 cells

FIGURE 6 |Uric acid induces SREBP-1c activation and JAK2/STAT3 pathway inhibition in vitro.F, p-JAK2 (Tyr1007/1008)/JAK2 and p-STAT3 (Tyr705)/STAT3 protein levels were detected by western blotting.
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