Phospho-DOK2 (Tyr299) Antibody - #AF3021
| 产品: | 磷酸化 DOK2 (Tyr299) 抗体 |
| 货号: | AF3021 |
| 描述: | Rabbit polyclonal antibody to Phospho-DOK2 (Tyr299) |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Dog, Xenopus |
| 分子量: | 56kDa; 45kD(Calculated). |
| 蛋白号: | O60496 |
| RRID: | AB_2834428 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3021, RRID:AB_2834428.
展开/折叠
Docking protein 2 56kDa; Docking protein 2; DOK 2; DOK R; DOK2; DOK2_HUMAN; Downstream of tyrosine kinase 2; OTTHUMP00000161710; OTTHUMP00000224923; p56(dok 2); p56(dok-2); p56dok 2; p56DOK; p56dok2;
抗原和靶标
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
- O60496 DOK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDGAVKQGFLYLQQQQTFGKKWRRFGASLYGGSDCALARLELQEGPEKPRRCEAARKVIRLSDCLRVAEAGGEASSPRDTSAFFLETKERLYLLAAPAAERGDWVQAICLLAFPGQRKELSGPEGKQSRPCMEENELYSSAVTVGPHKEFAVTMRPTEASERCHLRGSYTLRAGESALELWGGPEPGTQLYDWPYRFLRRFGRDKVTFSFEAGRRCVSGEGNFEFETRQGNEIFLALEEAISAQKNAAPATPQPQPATIPASLPRPDSPYSRPHDSLPPPSPTTPVPAPRPRGQEGEYAVPFDAVARSLGKNFRGILAVPPQLLADPLYDSIEETLPPRPDHIYDEPEGVAALSLYDSPQEPRGEAWRRQATADRDPAGLQHVQPAGQDFSASGWQPGTEYDNVVLKKGPK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60496 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K7 | Ubiquitination | Uniprot | |
| Y12 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| Y139 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| T144 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| T189 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | P08631 (HCK) , P07948 (LYN) , P12931 (SRC) | Uniprot |
| S277 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| Y299 | Phosphorylation | P08631 (HCK) , P07948 (LYN) , P12931 (SRC) | Uniprot |
| S309 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| Y330 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| Y345 | Phosphorylation | P07948 (LYN) , P08631 (HCK) , P12931 (SRC) | Uniprot |
| Y357 | Phosphorylation | Uniprot | |
| Y402 | Phosphorylation | Uniprot |
研究背景
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).
On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).
(Microbial infection) Interacts with Herpes simplex virus 1 (HHV-1) protein UL46; this interaction induces DOK2 phosphorylation and subsequent degradation.
PTB domain mediates receptor interaction.
Belongs to the DOK family. Type A subfamily.
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