YY1 Antibody - #DF8072

Figure 1 miR-141-3p and YY1 expression in a fetal rat model of ETU-induced ARMs (A) Gross morphology of fetal rats in the control and ARMs groups, observed under a stereomicroscope on E17 (arrow: no anus and tail in ARMs fetal rat; arrowhead: normal anus with tail; scale bar, 2 mm). (B) The expression patterns of miR-141-3p (red) detected by FISH assays in lumbosacral spinal cords of control and ARMs embryos, combined with nuclei staining using DAPI (blue) at 10× (scale bar, 50 μm) and 40× (scale bar, 12.5 μm) (oil). (C) Quantification of miR-141-3p expression by AOD analysis in lumbosacral spinal cords of control and ARMs embryos. (D) Relative miR-141-3p-expression levels in the lumbosacral spinal cords of normal rat fetuses and rat fetuses with ETU-induced ARMs on E17 by qRT-PCR (n = 8). (E) Colocalization of miR-141-3p (red) and NESTIN (green) in the spinal cord of fetal rat detected by combined FISH/immunostaining at 80× (scale bar, 6.25 μm). (F) Relative expression levels of Yy1 mRNA in the lumbosacral spinal cords of normal rat fetuses and rat fetuses with ETU-induced ARMs on E17 (n = 8). (G) Comparison of the protein-expression levels of YY1 in lumbosacral spinal cords from rat fetuses on the control and ARMs groups on E17 (n = 8). (H) Correlation analysis for miR-141-3p and Yy1 RNA-expression levels in rat fetuses with ARMs on E17. The data shown represent the results of 3 experiments. The data shown indicate the mean values of triplicate experiments ± SD. (N17, normal fetal rats on E17; A17: fetal rats with ARMs on E17.) ∗p < 0.05, ∗∗p < 0.01, relative to the control group.