产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8070, RRID:AB_2841425.
展开/折叠
Abelson interactor 2; ABI 2; Abi-2; ABI2; ABI2_HUMAN; ABI2B; Abl binding protein 3; Abl interacting protein 1 (SH3 containing protein); Abl interactor 2; Abl interactor protein 2b; Abl-binding protein 3; AblBP3; AIP 1; Arg binding protein 1; Arg protein tyrosine kinase binding protein; Arg-binding protein 1; ArgBP1; ARGBPIA; ArgBPIB; OTTHUMP00000163783; OTTHUMP00000163784; OTTHUMP00000206182; OTTHUMP00000206183; OTTHUMP00000206184; OTTHUMP00000206185; SSH3BP2;
抗原和靶标
Widely expressed. Abundant in testes, ovary, thymus, and colon, with lower but detectable levels in prostate, peripheral blood leukocytes, and spleen.
- Q9NYB9 ABI2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAELQMLLEEEIPGGRRALFDSYTNLERVADYCENNYIQSADKQRALEETKAYTTQSLASVAYLINTLANNVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANLERPVRYIRKPIDYTILDDIGHGVKWLLRFKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPSPTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSVFPAPAGSAGTPPLPATSASAPAPLVPATVPSSTAPNAAAGGAPNLADGFTSPTPPVVSSTPPTGHPVQFYSMNRPASRHTPPTIGGSLPYRRPPSITSQTSLQNQMNGGPFYSQNPVSDTPPPPPPVEEPVFDESPPPPPPPEDYEEEEAAVVEYSDPYAEEDPPWAPRSYLEKVVAIYDYTKDKEDELSFQEGAIIYVIKKNDDGWYEGVMNGVTGLFPGNYVESIMHYSE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NYB9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K43 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| R205 | Methylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| R230 | Methylation | Uniprot | |
| T231 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T361 | Phosphorylation | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| Y460 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| K466 | Acetylation | Uniprot | |
| K482 | Acetylation | Uniprot | |
| S512 | Phosphorylation | Uniprot |
研究背景
Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation. Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge. Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity). Also required for nascent adherens junction assembly in epithelial cells.
Phosphorylated by ABL1.
Cytoplasm. Nucleus.
Cell projection>Lamellipodium. Cell projection>Filopodium. Cytoplasm>Cytoskeleton. Cell junction>Adherens junction.
Note: Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips (PubMed:11516653, PubMed:15572692). Present at nascent adherens junctions, where it clusters adjacent to the tips of F-actin protrusions (PubMed:15572692).
Widely expressed. Abundant in testes, ovary, thymus, and colon, with lower but detectable levels in prostate, peripheral blood leukocytes, and spleen.
Component of the WAVE complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. Interacts (via SH3 domain) with ABL1 and ABL2.
(Microbial infection) Interacts with human cytomegalovirus UL135.
The SH3 domain is critical for binding to ABL1 and ABL2.
Belongs to the ABI family.
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
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