RAD51 Antibody - #DF8066

Figure3.SP8 promotes chemotherapy resistance in small cell lung cancer through homologous recombination repair pathways A-C. RNA expression difference analysis was performed after interfering SP8 expression in H69AR cells ,n=3. Volcano plot and KEGG analysis and biological process analysis were performed on the results of RNA-sequencing analysis. D. After interfering with SP8 expression in H69AR, H446DDP, respectively, western blot was used to detect the protein expression level of PALB2 E. H69AR and H446DDP were exposed to cisplatin and etoposide with their IC50 values, respectively, after interference of SP8 expression, and the protein levels of PALB2 and RAD51 were measured by Western blot F. H69AR and H446DDP were exposed to cisplatin with their IC50 values, and the effects of interference with SP8 expression on the degree of trailing were observed by alkaline comet assay. G. A statistical of the percentage of tailing DNA H,I. H69AR was exposed to cisplatin and etoposide with IC50 value, andcthe change of γH2AX intracellular density after interference of SP8 expression was observed by immunofluorescence L. H69AR and H446DDP were exposed to cisplatin with their IC50 values, and the homologous recombination repair was observed by transferring into I-SCEl and DRreport plasmids K. H69AR and H446DDP were exposed to Talazoparib of their IC50 value, and the homologous recombination repair was observed by transferring into I-SCEl and DRreport plasmids M. The binding of endogenous SP8 and homologous recombination repair related proteins was observed by CO-IP assay