SLC7A5 Antibody - #DF8065

Figure 4 p-AKT promoted lipid metabolism and nutrient absorption in the intestine of Aurkaf/+;VillinCre/+ mice. (A, B) Lipid metabolism- and nutrient absorption-related mRNA and protein levels of were measured in the intestines of Aurkaf/+;VillinCre/+ mice. The graphs show the mean ± SEM of one of three independent experiments. (n=3 mice per group). *, P < 0.05. **, P <0.01. (C, D, E, F) The relative length and width of crypts and villi were measured by ImageJ software. The histogram shows the mean ± SEM. (G) The ratio of villi to crypt length in the intestine. (H, I) The concentrations of IL-6 and IL-1β in the serum were measured by ELISA. The bar graphs show the mean ± SEM of one of two independent experiments that included five groups of mice. *P <0.05. (J, K) H&E staining and CD3 immunostaining of the intestines of Aurkaf/+and Aurkaf/+;VillinCre/+ mice. The quantitative data are shown on the right.

FIGURE 3 Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.

Figure 1 Representative immunoblots of ZnT1, ZnT4, ZnT5, ZnT7, ZnT9, ZIP3, ZIP5, B0AT1, LAT1, y+LAT2, rBAT and PepT1 in the jejunum of broilers at 28 (A) and 39 d (B) of age. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y+LAT2, y+L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; ZnS, Zn sulfate; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6).

Fig. 4. |The uptake of 4-[18F]FGln reduced in ASCT2 knockdown tumors and cells and analysis of ASCT2 and LAT1 proteins after shRNA knockdown. a 4-[18F]FGln uptake in shASCT2-IMR-32 showed lower uptake than that in shControl-IMR-32, while[18F]FDG uptake showed no significant difference during the 120 min. b Relative mRNA expression of ASCT2 was calculated by 2−△△Ct method, and a reduced expression was observed after shASCT2 knockdown; ASCT2 expression showed a significant difference between in shASCT2 and shControl cells and tumor tissues. cASCT2 knockdown had no significant effect on the expression of LAT1 protein.

Figure 2. Effect of Zn source (added Zn level of 400 μmol/L) on protein expression levels of ZnT, ZIP, amino acid, and small peptide transporters in primary cultured duodenal epithelial cells at 120 min of incubation (experiment 3). Values are means ± SE (n = 5 to 6). Lacking the same letters (a, b, c) means significant differences (P