Ras-GRF1 Antibody - #DF8026

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产品: Ras-GRF1 抗体
货号: DF8026
描述: Rabbit polyclonal antibody to Ras-GRF1
应用: WB IF/ICC
文献验证: WB
反应: Human
预测: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
蛋白号: Q13972
RRID: AB_2841395

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
Ras-GRF1 Antibody detects endogenous levels of total Ras-GRF1.
RRID:
AB_2841395
引用格式: Affinity Biosciences Cat# DF8026, RRID:AB_2841395.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDC25; CDC25L; GNRP; GRF1; GRF55; Guanine nucleotide exchange factor; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein; guanine nucleotide-releasing factor 1; Guanine nucleotide-releasing protein; H GRF55; HGRF55; PP13187; Ras protein specific guanine nucleotide releasing factor 1; Ras specific guanine nucleotide releasing factor; Ras specific guanine nucleotide releasing factor CDC25 homolog; Ras specific nucleotide exchange factor CDC25; Ras-GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-specific nucleotide exchange factor CDC25; RASGRF1; RGRF1_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Ras-GRF1, corresponding to a region within N-terminal amino acids.

基因/基因ID:
RASGRF1(5923)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

文献引用

1). Thrombospondin 1 Promotes Cytoskeleton Remodeling, Dedifferentiation, and Pulmonary Metastasis through ITGA1 and ITGA6 in Osteosarcoma. International journal of biological sciences, 2025 (PubMed: 40083708) [IF=8.2]

Application: WB    Species: human    Sample: osteosarcoma cell

Figure 3 The role and mechanism of THBS1 in osteosarcoma cell dedifferentiation and tumor malignancy. (A) Wound-healing assay results of THBS1 knockdown and overexpression cells are shown. (B) Transwell migration and invasion assay results of THBS1 knockdown and overexpression cells are shown. (C) Cell morphological changes after expression of THBS1 in different osteosarcoma cell lines are shown. Cells were stained for F-actin (magenta), DAPI (blue). (D) The expression of p-FAK, FAK, p-RasGRF1, RasGRF1, p-MLC2 and MLC2 in THBS1 knockdown and overexpression cells was determined. (E) The expression of GTP-RhoA and total RhoA in each group was shown.

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