产品: OLIG2 抗体
货号: DF8004
描述: Rabbit polyclonal antibody to OLIG2
应用: WB IHC
文献验证: WB, IHC
反应: Human, Monkey
预测: Pig, Dog, Chicken, Xenopus
蛋白号: Q13516
RRID: AB_2841383

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Monkey
克隆:
Polyclonal
特异性:
OLIG2 Antibody detects endogenous levels of total OLIG2.
RRID:
AB_2841383
引用格式: Affinity Biosciences Cat# DF8004, RRID:AB_2841383.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Basic domain helix loop helix protein class B 1; Basic helix loop helix protein class B 1; BHLHB; bHLHB1; bHLHe19; Class B basic helix loop helix protein 1; Class B basic helix-loop-helix protein 1; class E basic helix loop helix protein 19; Class E basic helix-loop-helix protein 19; Human protein kinase C binding protein RACK17; Olig2; OLIG2_HUMAN; Oligo2; Oligodendrocyte lineage transcription factor 2; Oligodendrocyte specific bHLH transcription factor 2; Oligodendrocyte transcription factor 2; OTTHUMP00000067569; OTTHUMP00000067570; PRKCBP2; Protein kinase C binding protein 2; Protein kinase C binding protein RACK17; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; RACK17;

抗原和靶标

免疫原:

A synthesized peptide derived from human OLIG2, corresponding to a region within the internal amino acids.

基因/基因ID:

文献引用

1). ACT001 inhibited CD133 transcription by targeting and inducing Olig2 ubiquitination degradation. Oncogenesis, 2023 (PubMed: 36990974) [IF=5.9]

Application: WB    Species: human    Sample: lung cancer tissues

Fig. 1: Olig2 was overexpressed in lung cancer tissues. a The representative pictures of immunohistochemical staining of Olig2 in paraffin-embedded human lung cancer tissues and non-lung cancer tissues. b Statistical analysis of Olig2 immunohistochemical staining of lung cancer tissues and non-lung cancer tissues, n = 8. c Western blot analysis of Olig2 expression in lung cancer tissues and non-lung cancer tissues, n = 8. d Statistical analysis of Olig2 expression in lung cancer tissues and non-lung cancer tissues, values were normalized to β-actin, n = 8. The data were presented as the mean ± SD, *p 

Application: IHC    Species: human    Sample: lung cancer tissues

Fig. 2: Olig2 promoted lung cancer cell stemness. a Average correlations between Olig2 expression and the functional states in glioma. b Representative images and number of tumorspheres with Olig2 knockdown or overexpression in NCI-H820 cells, n = 6. c Representative images and number of NCI-H820 migrated cells with Olig2 knockdown or overexpression, n = 3. d The bioluminescence imaging of lungs with intravenous injection of NCI-H820-luci cells into BAL B/c nude mice for 7 days, n = 5. e Statistical analysis of bioluminescence intensity in lungs, n = 5. f Representative HE staining images of lung and liver metastasis. g The picture of xenograft tumors derived from NCI-H820 cells with intra-tumoral injected Olig2 siRNA or si-NC in lipofectamine 2000. h Xenograft tumor growth curve of Olig2 siRNA or si-NC-transfected group, n = 4. i Xenograft tumor weight of Olig2 siRNA or si-NC-transfected group, n = 4. j Immunohistochemical staining of Olig2 and CD133 of xenograft tumor. k Western bolt analysis of Olig2, CD133, Nanog, and Oct4 expression in xenograft tumors. l Statistical analysis of Olig2, CD133, Nanog, and Oct4 expression in xenograft tumors, values were normalized to β-actin, n = 4. The data were presented as the mean ± SD, *p 

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.