ACTN4 Antibody - #DF8000

FIGURE 3 Phosphorylase kinase regulatory subunit alpha 1 antisense RNA 1 (PHKA1-AS1) was found to localize in the cytoplasm and interact with actinin alpha 4 (ACTN4). (A) PHKA1-AS1 and cell nucleus were labeled with PHKA1-AS1 specific fluorescent probe (red) and Hoechst33342 dye (blue), respectively. The subcellular localization of PHKA1-AS1 was detected by fluorescence in situ hybridization (FISH) assay (scale bar for large photos = 50 µm; scale bar for small photos = 10 µm). (B) Silver staining was performed on the RNA pull-down protein (red arrow: position of target protein ACTN4). (C) After mass spectral analysis of the pull-down proteins in the anti-sense group and the sense group, the intersecting Venn diagram was taken. (D) Subcellular distribution analysis (https://wolfpsort.hgc.jp/) of RNA pull-down proteins and functional annotation of protein Gene Ontology (GO). (E) The interaction between PHKA1-AS1 and ACTN4 was demonstrated by RNA immunoprecipitation (RIP) assay. ***p < 0.001, n = 3. (F) Western blotting assay was used to detect the expression of ACTN4 after up-regulating or down-regulating PHKA1-AS1.

Figure 4. Validity verification of DEDRGs. (A) Validation of DEDRGs by Western blotting. (B) Statistical plots of SLC7A11, ACTB, ACTN4, INF2, and MYL6. Compared with the saline group, ns = no significance, * p < 0.05, ** p < 0.01. n = 3. (C) Location of ACTN4 and INF2 proteins in cells from the HPA database: green represents the target protein, red represents microtubules, yellow represents the endoplasmic reticulum, and blue represents the nucleus (scale bar, 20 µm).