产品: APOD 抗体
货号: DF7987
描述: Rabbit polyclonal antibody to APOD
应用: WB IF/ICC
文献验证: IF/ICC
反应: Human, Mouse, Rat
分子量: 30 kDa; 21kD(Calculated).
蛋白号: P05090
RRID: AB_2841371

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 50ul RMB¥ 1250 现货
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
APOD Antibody detects endogenous levels of total APOD.
RRID:
AB_2841371
引用格式: Affinity Biosciences Cat# DF7987, RRID:AB_2841371.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

APO D; Apo-D; ApoD; APOD protein; APOD_HUMAN; Apolipoprotein D; ApolipoproteinD;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P05090 APOD_HUMAN:

Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.

序列:
MVMLLLLLSALAGLFGAAEGQAFHLGKCPNPPVQENFDVNKYLGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYWILATDYENYALVYSCTCIIQLFHVDFAWILARNPNLPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLS

研究背景

功能:

APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

翻译修饰:

N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor).

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.

亚基结构:

Homodimer. In plasma, also exists as a disulfide-linked heterodimer with APOA2.

蛋白家族:

Belongs to the calycin superfamily. Lipocalin family.

文献引用

1). Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. Nature Communications, 2022 (PubMed: 35508470) [IF=16.6]

Application: IF/ICC    Species: Mouse    Sample: articular cartilage

Fig. 5 scRNA-seq analysis identified three subsets of chondrocytes. a Medial femoral condyle tissue from the region enclosed by the dotted lines was isolated for cell dissociation. n = 9 mice. Scale bar, 250 μm. b Putative chondrocyte population (171 cells) and unsupervised clustering identified clusters C1, C2, and C3 in cartilage. c Heatmap showing the expression of the top 20 identified significantly differentially expressed genes in clusters C1, C2, and C3. d Immunofluorescence staining of representative markers identified by scRNA-seq in articular cartilage. n = 3 independent experiments. All scale bars, 100 μm. e GO analysis and representative genes for clusters C1, C2, and C3.

2). Identifying the signature of NAD+ metabolism-related genes for immunotherapy of gastric cancer. Heliyon, 2024 (PubMed: 39640811) [IF=4.0]

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