(2)
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| 产品: | ATG12 抗体 |
| 货号: | DF7937 |
| 描述: | Rabbit polyclonal antibody to ATG12 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 22 kDa; 15kD(Calculated). |
| 蛋白号: | O94817 |
| RRID: | AB_2841346 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7937, RRID:AB_2841346.
展开/折叠
APG12-like; APG12L; ATG12; ATG12 autophagy related 12 homolog (S. cerevisiae); ATG12 autophagy related 12 homolog; ATG12_HUMAN; Autophagy 12; Autophagy-related protein 12; FBR93; HAPG12; Ubiquitin-like protein ATG12;
抗原和靶标
- O94817 ATG12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O94817 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S41 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| K89 | Ubiquitination | Uniprot |
研究背景
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.
(Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established.
Acetylated by EP300.
Cytoplasm. Preautophagosomal structure membrane>Peripheral membrane protein.
Note: TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.
Ubiquitous.
Forms a conjugate with ATG5. The ATG12-ATG5 conjugate forms a complex with several units of ATG16L. Interacts with DHX58/RIG-1, IFIH1/MDA5 and MAVS/IPS-1 in monomeric form as well as in ATG12-ATG5 conjugate. The interaction with MAVS is further enhanced upon vesicular stomatitis virus (VSV) infection. Interacts with ATG3 and ATG7. Interacts with ATG10 (By similarity). Interacts with TECPR1.
Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
Belongs to the ATG12 family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
文献引用
Application: WB Species: Mouse Sample: brain tissue
Application: WB Species: Rat Sample: cartilage tissue
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