p53 Antibody - #AF0865

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产品: p53 抗体
货号: AF0865
描述: Rabbit polyclonal antibody to p53
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
蛋白号: P04637
RRID: AB_2834414

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IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
p53 Antibody detects endogenous levels of total p53.
RRID:
AB_2834414
引用格式: Affinity Biosciences Cat# AF0865, RRID:AB_2834414.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Antigen NY-CO-13; BCC7; Cellular tumor antigen p53; FLJ92943; LFS1; Mutant tumor protein 53; p53; p53 tumor suppressor; P53_HUMAN; Phosphoprotein p53; Tp53; Transformation related protein 53; TRP53; Tumor protein 53; Tumor protein p53; Tumor suppressor p53;

抗原和靶标

免疫原:

A synthesized peptide derived from human p53, corresponding to a region within N-terminal amino acids.

基因/基因ID:
TP53(7157)
描述:
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Morusin Alleviates Aortic Valve Calcification by Inhibiting Valve Interstitial Cell Senescence Through Ccnd1/Trim25/Nrf2 Axis. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38502885) [IF=15.1]
2). Di-2-ethylhexyl phthalate (DEHP) exposure induces sperm quality and functional defects in mice. Chemosphere, 2023 (PubMed: 36372335) [IF=8.1]
3). Sulfur dioxide and benzo(a)pyrene trigger apoptotic and anti-apoptotic signals at different post-exposure times in mouse liver. Chemosphere, 2015 (PubMed: 26162325) [IF=8.1]

Application: WB    Species: mouse    Sample: mouse liver

Fig. 4. Effects of SO2 and/or BaP on protein expression in mouse livers. (A), Representative bands of mouse liver proteins after chlorpyrifos exposure. Proteins (50 lg) obtained from mouse liver after post-exposure to SO2 and/or BaP for 1 d or 13 weeks were used to blot. GAPDH was used as a loading control. (B–H), The protein level of cleaved casepase-3 (B), cleaved caspase-9 (C), bcl-2 (D), bax (E), bcl-2/bax ratio (F), p53 (G), and p53 phosphorylation on Ser15 (H) were measured by densitometric analysis and normalized to the loading control (n = 1). Each column and bar represents the mean ± SE of at least six individual sample; ⁄ P < 0.05, ⁄⁄P < 0.01, ⁄⁄⁄P < 0.001 versus control group; #P < 0.05, ##P < 0.01, ###P < 0.001 versus SO2 alone; a P < 0.05, b P < 0.01, c P < 0.001 versus BaP alone.
4). PLAC8 attenuates pulmonary fibrosis and inhibits apoptosis of alveolar epithelial cells via facilitating autophagy. Communications biology, 2025 (PubMed: 39810019) [IF=5.9]
5). miR‑29b suppresses proliferation and induces apoptosis of hepatocellular carcinoma ascites H22 cells via regulating TGF‑β1 and p53 signaling pathway. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2021 (PubMed: 34184070) [IF=5.7]

Application: WB    Species: Mice    Sample: NCTC1469, Hepa1-6 and H22 cells

Figure 1 Expression levels of miR-29b, TGF-β1 and p53 in NCTC1469, Hepa1-6 and H22 cells. (A) miR-29b expression. Values represent the means ± SD (n=6). (B) Western blot analysis of TGF-β1 and p53. (C and D) The intensity of TGF-β1 and p53 relative to β-actin. Values represent the means ± SD (n=3). *P<0.05 and **P<0.01 vs. NCTC1469. miR, microRNA; TGF, transforming growth factor; H22, hepatoma 22; SD, standard deviation.
6). The mTOR promotes oxidative stress-induced apoptosis of mesangial cells in diabetic nephropathy. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2018 (PubMed: 29277549) [IF=3.8]
7). Nonlinear ageing gero-marker dynamics of transcriptomic profile during calcific aortic valve mouse modeling. Archives of gerontology and geriatrics, 2025 (PubMed: 39922128) [IF=3.5]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 1. Aortic function changes during ageing process in mouse model. (A) Representative images of PW doppler mode echocardiography of mice from each group. (B) Representative images of M-mode echocardiography of mice from each group. (C) Representative images of HE-stained cardiac tissues of mice from each group. (D) Representative images of SR-stained cardiac tissues of mice from each group through polarization microscope. (E) Pvel based on echocardiographic measurements, n = 3. (F) MPG based on echocardiographic measurements, n = 3. (G) AVD based on echocardiographic measurements, n = 3. (H) The area of collagenous fiber I and III measured according to SR staining, n = 3.
8). Prognostic value of p16, p53, and pcna in sarcoma and an evaluation of immune infiltration. Journal of Orthopaedic Surgery and Research, 2022 (PubMed: 35689249) [IF=2.8]

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