APOA2 Antibody - #DF7905

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产品: APOA2 抗体
货号: DF7905
描述: Rabbit polyclonal antibody to APOA2
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Dog
分子量: 11 kDa; 11kD(Calculated).
蛋白号: P02652
RRID: AB_2841322

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(80%), Sheep(80%), Dog(83%)
克隆:
Polyclonal
特异性:
APOA2 Antibody detects endogenous levels of total APOA2.
RRID:
AB_2841322
引用格式: Affinity Biosciences Cat# DF7905, RRID:AB_2841322.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

APO A2; Apo AII; Apo-AII; APOA 2; ApoA II; ApoA-II; APOA2; APOA2_HUMAN; APOAII; Apolipoprotein A II; Apolipoprotein A-II(1-76); Apolipoprotein A2; Apolipoprotein AII; ApolipoproteinA II; OTTHUMP00000032244;

抗原和靶标

免疫原:
Uniprot:

P02652(APOA2_HUMAN) >>Visit HPA database.

基因/基因ID:
APOA2(336)
表达:
P02652 APOA2_HUMAN:

Plasma; synthesized in the liver and intestine.

序列:
MKLLAATVLLLTICSLEGALVRRQAKEPCVESLVSQYFQTVTDYGKDLMEKVKSPELQAEAKSYFEKSKEQLTPLIKKAGTELVNFLSYFVELGTQPATQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
83
Bovine
80
Sheep
80
Rabbit
75
Horse
67
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P02652 作为底物

Site PTM Type Enzyme
S32 Phosphorylation
S35 Phosphorylation
Y37 Phosphorylation
T42 O-Glycosylation
S54 Phosphorylation
K67 Acetylation
S68 Phosphorylation

研究背景

功能:

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

翻译修饰:

Phosphorylation sites are present in the extracellular medium.

Apolipoprotein A-II is O-glycosylated.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Plasma; synthesized in the liver and intestine.

亚基结构:

Monomer. Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD.Interacts with APOA1BP and NDRG1.

(Microbial infection) Interacts with HCV core protein.

蛋白家族:

Belongs to the apolipoprotein A2 family.

研究领域

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Digestive system > Cholesterol metabolism.

文献引用

1). Neural stem cell-derived exosomes regulate neural stem cell differentiation through miR-9-Hes1 axis. Frontiers in Cell and Developmental Biology (PubMed: 34055767) [IF=5.5]

Application: WB    Species: Mouse    Sample: neural stem cells (NSC)

FIGURE 1 Characterization of exosomes (EXOs). (A) Purified exosomes were observed under transmission electron microscopy (TEM) using negative staining. (B) The levels of positive exosomal markers TSG101, CD9, and Flotillin-1 in protein lysates of neural stem cells (NSC)-derived exosome pellets were determined by western blotting. (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting. (D,E) Particle-size distribution of exosomes was determined by NanoSight analysis (NTA) in panel (D) and NanoFCM in panel (E) technologies. Scale bar 200 nm in panel (A).

Application: WB    Species: mouse    Sample: NSCs

FIGURE 1 | Characterization of exosomes (EXOs). (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting.
2). Neural stem cell-derived exosomal FTO protects neuron from microglial inflammatory injury by inhibiting microglia NRF2 mRNA m6A modification. Journal of neurogenetics (PubMed: 37812019) [IF=1.9]

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