NRP1 Antibody - #DF7877

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产品: NRP1 抗体
货号: DF7877
描述: Rabbit polyclonal antibody to NRP1
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: O14786
RRID: AB_2841305

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
NRP1 Antibody detects endogenous levels of total NRP1.
RRID:
AB_2841305
引用格式: Affinity Biosciences Cat# DF7877, RRID:AB_2841305.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A5 protein; BDCA4; BLOOD DENDRITIC CELL ANTIGEN 4; CD304; Neuropilin-1; Neuropilin1; NP1; NPN1; NRP 1; NRP; NRP1; NRP1_HUMAN; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R;

抗原和靶标

免疫原:

A synthesized peptide derived from human NRP1, corresponding to a region within the internal amino acids.

基因/基因ID:
NRP1(8829)

研究领域

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). Neuropilin-1 Interacts with Fibronectin-1 to Promote Epithelial–Mesenchymal Transition Progress in Gastric Cancer. OncoTargets and Therapy, 2020 (PubMed: 33116644) [IF=2.7]

Application: WB    Species: human    Sample: gastric cancer cells

Figure 3 |Establishment of NRP1 overexpression and knockdown cell lines (A) Analysis of NRP1 expression in different cell lines using Western blot.

Application: WB    Species: Human    Sample: gastric cancer tissue

Figure 1 NRP1 highly expressed in gastric cancer and predicts poor prognosis. (A–B) Survival curve of high NRP1 expression in patients with gastric cancer. (C) According to the TCGA database, high expression of NRP1 in gastric cancer tissues compared with normal tissues. (D) The high expression of NRP1 in metastatic gastric cancer. (E) Expression of NRP1 in gastric cancer patients with different pathological grade. (F) Expression of NRP1 in gastric cancer patients of different clinical stage. (G) Protein levels of NRP1 in ten pairs of fresh tumor tissues and adjacent normal tissues. Differences were considered significant at P < 0.05 and labeled with *.

Application: IHC    Species: Human    Sample: gastric cancer cell

Figure 3 Establishment of NRP1 overexpression and knockdown cell lines (A) Analysis of NRP1 expression in different cell lines using Western blot. (B) Detection of protein levels of NRP1 in gastric cancer cell lines after NRP1 overexpression of knockdown. (C) Wound healing was used to detect cell migration after ectopic alteration of NRP1 expression. (D) Transwell was performed to detect cell invasion after ectopic alteration of NRP1 expression. (E) Cell proliferation was analyzed by colony formation. (F) Protein levels of E-cadherin, N-cadherin and Vimentin were detected by Western blot. Differences were considered significant at P < 0.05 and labeled with *

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