GAP43 Antibody - #DF7766

Fig. 5. |Effects of SNS on synaptic-associated protein in the HIP and PFC of stressed rats. Representative immunoblots for PSD-95, GAP-43, Syn, and Tublin in the HIP(A) and PFC(B) regions. (A) Results of relative protein levels of PSD-95, GAP-43, and Syn in the HIP of rats of each group.

Figure 3 Effect of iPSC-NPC-derived exosomes (OGD+iNPC-exo) on expression of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins in OGD induced neurons. (A–C) mRNA expression (A) and protein expression (B, C) of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins (NF200, GAP-43, Synapsin, and PSD95) analyzed by polymerase chain reaction and western blot assay. The mRNA expression is described by the optical density ratio relative to the control group. Protein expression was described by the optical density ratio relative to β-actin. Data are presented as mean ± SD. ***P < 0.001, vs. control group; ###P < 0.001 (one-way analysis of variance with post hoc Bonferroni test). All experiments were repeated three times. GAP43: growth associated protein 43; iPSC-NPCs: induced pluripotent stem cells-derived neural progenitor cells; NF200: neurofilament 200; OGD: oxygen-glucose deprivation; p-Akt: phosphor-Akt; PSD95: postsynaptic density protein 95; PTEN: phosphatase and tensin homolog deleted on chromosome ten.

Figure 5 MS reduces the expression of synaptic-plasticity protein. (A) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in the hippocampus by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. (B) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in cortex by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. Statistical analyses are performed by two-way ANOVA followed by t-test. Data are presented as mean ± SEM, *p < 0.05, **p < 0.01, n = 3 per group.

Fig. 6 |EPO benefit antiinflammation and axonal regeneration can be neutralized by ERK inhibition, and activator can mimic the effect of EPO. a The axonal special protein GAP43 immunofluorescence staining and histogram at 14 days (magnification × 400). b–e The inflammatory marker proteins immunofluorescence staining and histograms at 14 days, including CD86, GFAP, TNF-α, iNOS(magnification × 400). All data presented as mean ± SEM in each group. *p < 0.05 vs. SCI + saline group, **p < 0.01 vs. SCI + saline group. #p < 0.05 comparison between SCI + EPO group and SCI + EPO + PD98059, ##p < 0.01 comparison between SCI + EPO group and SCI + EPO + PD98059

Fig. 3. Validation of GAP-43 expression in pancreatic cancer samples, data were obtained from the authors' affiliations. (A) Immunohistochemical results showed GAP-43 expression in PNI, normal pancreatic tissues, and pancreatic cancer tissues with non-PNI. Scale bar: 200 μm. (B) The IOD value of GAP-43 staining in PNI, normal tissues, and tumors without PNI. (C) IHC scores differed between tumor and tumor, and between PNI and non-PNI in the tumor, respectively. (D) Survival difference between high and low GAP-43 expression in pancreatic cancer. (D) Immunohistochemical staining of GAP-43(−), GAP-43(+), GAP-43(2+), GAP-43(3+). ****p < 0.0001.

Fig. 5. Validation of probe targeting and toxicity in vitro. (A) Confocal microscopy images of PC12, Miapaca-2 and PC12* after incubation with GAP-43Ra-ICG-PEG or ICG-NP. Magnification:630×. (B) CCK-8 results in probes GAP-43Ra-ICG-PEG and ICG-NP. (C) Fluorescence quantitative differences of GAP-43Ra-ICG-PEG or ICG-NP uptake by PC12, Miapaca-2 and PC12* under confocal microscopy. (D) GAP-43 expression of PC12 and PC12* in western blot. (F) Quantitative analysis of PC12 and PC12*in western blot. PC12*: PC12 cells after co-culture with pancreatic cancer cell line Miapaca-2.****p < 0.0001.

Fig. 9 Effects of TAG-1 treatment on the GAP-43 and NCAM expression in the rats with SNI. A The expression of GAP-43 was measured by immunohistochemistry. Scale = 50 μm. Black arrow: GAP-43 expression. The expression of GAP-43 was analyzed using ImageJ software. B The expression of NCAM was measured by immunofluorescence. The mean gray of NCAM was analyzed by ImageJ software. *p 

Figure 5 Western blot analysis. The expressions of synaptic plasticity proteins were determined by western blot (A), including PSD-95 (B), GAP-43 (C), and SYN (D). The values represent the mean ± SEM, n = 5. *p < 0.05, **p < 0.01 vs. CON, #p < 0.05, ##p < 0.01 vs. MS. CON, control; MS, maternal separation; CUMS, chronic unpredictable mild stress.