IGFBP2 Antibody - #DF7765

Fig. 3. The single-cell transcriptomes of epithelial cells in non-malignant and malignant esophagus.(a) The TSNE plot and overview of epithelial cells by the cell type and origin of the cells. (b) Canonical cell markers were used to label epithelial subtypes as represented in the TSNE plot. (c) The TSNE plot of epithelial cells by the CNV’s levels of the cells. (d) Heatmap showing the expression of marker genes in malignant and non-malignant epithelial cells. (e) Violin plots showing the marker genes for each epithelial cell type. (f) Immunofluorescence staining of tumor (n = 8) (up) / normal (n = 8) (down) tissue-specific genes in ESCC and non-malignant tissues. Comparison of non-malignant and malig- nant esophagus was performed by Wilcoxon test. (g) Pseudotime analysis for malignant and non-malignant epithelial cells. (h) Differences in pathway activities scored per cell by GSVA between malignant and non-malignant epithelial cells. The scores of pathways are normalized.

Figure 5.Identification of growth-factor secretion in human γδ T+ Cells. (A) qRT-PCR results from eight selected genes using RNA-sequencing. (B) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from early decidua (n = 5). (C) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from term decidua (n = 5). (D) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from peripheral blood mononuclear cells (PBMCs) (n = 5). (E) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from recurrent spontaneous abortion (RSA) patients (n = 5; scale bar, 50 μm). (F) qRT-PCR results regarding growth factors in sorted γδ T cells from RSA patients (n = 5). P-value was calculated by unpaired Student’s t-test.