F4/80 Antibody - #DF7762

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产品: F4/80 抗体
货号: DF7762
描述: Rabbit polyclonal antibody to F4/80
应用: WB IF/ICC
文献验证:
反应: Human
预测: Bovine, Sheep
分子量: 102 kDa; 98kD(Calculated).
蛋白号: Q14246
RRID: AB_2841228

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Bovine(83%), Sheep(83%)
克隆:
Polyclonal
特异性:
F4/80 Antibody detects endogenous levels of total F4/80.
RRID:
AB_2841228
引用格式: Affinity Biosciences Cat# DF7762, RRID:AB_2841228.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADGRE1; Adhesion G protein coupled receptor E1; Adhesion G protein-coupled receptor E1; AGRE1_HUMAN; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1 hormone receptor; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3;

抗原和靶标

免疫原:
Uniprot:

Q14246(AGRE1_HUMAN) >>Visit HPA database.

基因/基因ID:
ADGRE1(2015)
表达:
Q14246 AGRE1_HUMAN:

Expression is restricted to eosinophils.

序列:
MRGFNLLLFWGCCVMHSWEGHIRPTRKPNTKGNNCRDSTLCPAYATCTNTVDSYYCACKQGFLSSNGQNHFKDPGVRCKDIDECSQSPQPCGPNSSCKNLSGRYKCSCLDGFSSPTGNDWVPGKPGNFSCTDINECLTSSVCPEHSDCVNSMGSYSCSCQVGFISRNSTCEDVDECADPRACPEHATCNNTVGNYSCFCNPGFESSSGHLSFQGLKASCEDIDECTEMCPINSTCTNTPGSYFCTCHPGFAPSNGQLNFTDQGVECRDIDECRQDPSTCGPNSICTNALGSYSCGCIAGFHPNPEGSQKDGNFSCQRVLFKCKEDVIPDNKQIQQCQEGTAVKPAYVSFCAQINNIFSVLDKVCENKTTVVSLKNTTESFVPVLKQISTWTKFTKEETSSLATVFLESVESMTLASFWKPSANITPAVRTEYLDIESKVINKECSEENVTLDLVAKGDKMKIGCSTIEESESTETTGVAFVSFVGMESVLNERFFKDHQAPLTTSEIKLKMNSRVVGGIMTGEKKDGFSDPIIYTLENIQPKQKFERPICVSWSTDVKGGRWTSFGCVILEASETYTICSCNQMANLAVIMASGELTMDFSLYIISHVGIIISLVCLVLAIATFLLCRSIRNHNTYLHLHLCVCLLLAKTLFLAGIHKTDNKMGCAIIAGFLHYLFLACFFWMLVEAVILFLMVRNLKVVNYFSSRNIKMLHICAFGYGLPMLVVVISASVQPQGYGMHNRCWLNTETGFIWSFLGPVCTVIVINSLLLTWTLWILRQRLSSVNAEVSTLKDTRLLTFKAFAQLFILGCSWVLGIFQIGPVAGVMAYLFTIINSLQGAFIFLIHCLLNGQVREEYKRWITGKTKPSSQSQTSRILLSSMPSASKTG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
83
Sheep
83
Dog
67
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14246 作为底物

Site PTM Type Enzyme
T50 Phosphorylation
S53 Phosphorylation
Y736 Phosphorylation
S766 Phosphorylation
T770 Phosphorylation

研究背景

功能:

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expression is restricted to eosinophils.

蛋白家族:

Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.

文献引用

1). Huanglian Jiedu Decoction ameliorates DSS-induced colitis in mice via the JAK2/STAT3 signalling pathway. Chinese Medicine, 2020 (PubMed: 32411291) [IF=5.3]
2). Paracrine effect of the stromal vascular fraction containing M2 macrophages on human chondrocytes through the Smad2/3 signaling pathway. Journal of Cellular Physiology, 2022 (PubMed: 35766589) [IF=4.5]
3). Pioglitazone decreased renal calcium oxalate crystal formation by suppressing M1 macrophage polarization via the PPAR-γ-miR-23 axis. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2019 (PubMed: 31091119) [IF=3.7]

Application: IHC    Species: mouse    Sample: kidneys

Fig. 2.| Evaluation of expression of peroxisome proliferator-activated receptor (PPAR)-,macrophage (Mp)-related molecules, proinflammatory cytokines, and anti-inflammatory cytokines. A: immunohistochemical distribution of the expression of PPAR-, proinflammatory cytokines, and Mp-related molecules in the kidneys as well as the ratio of areas with positive expression. PPAR- expression in pioglitazone (PGZ)-treated groups was significantly higher than in other groups. Expression of monocyte chemotactic protein (MCP)-1, F4/80, inducible nitric oxide synthase (iNOS), arginine 1 (ARG1), interferon regulatory factor 1 (IRF1), and PKNOX1 was significantly higher in 3-day (d) glyoxylic acid (GA)- and 6-day GA-treated groups than in the control group and was significantly lower in PGZ-treated groups than in the 6-day GA group. Original magnification: 200.
4). Rosiglitazone Suppresses Renal Crystal Deposition by Ameliorating Tubular Injury Resulted from Oxidative Stress and Inflammatory Response via Promoting the Nrf2/HO-1 Pathway and Shifting Macrophage Polarization. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34691355)

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