BMP9 Antibody - #DF7758

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产品: BMP9 抗体
货号: DF7758
描述: Rabbit polyclonal antibody to BMP9
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Horse, Sheep, Rabbit
分子量: 47 kDa; 47kD(Calculated).
蛋白号: Q9UK05
RRID: AB_2841224

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Horse(89%), Sheep(%), Rabbit(%)
克隆:
Polyclonal
特异性:
BMP9 Antibody detects endogenous levels of total BMP9.
RRID:
AB_2841224
引用格式: Affinity Biosciences Cat# DF7758, RRID:AB_2841224.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BMP 9; Bone morphogenetic protein 9; GDF 2; GDF2; growth differentiation factor 2; Growth/differentiation factor 2; HHT5;

抗原和靶标

免疫原:
Uniprot:

Q9UK05(GDF2_HUMAN) >>Visit HPA database.

基因/基因ID:
GDF2(2658)
表达:
Q9UK05 GDF2_HUMAN:

Detected in blood plasma (at protein level).

序列:
MCPGALWVALPLLSLLAGSLQGKPLQSWGRGSAGGNAHSPLGVPGGGLPEHTFNLKMFLENVKVDFLRSLNLSGVPSQDKTRVEPPQYMIDLYNRYTSDKSTTPASNIVRSFSMEDAISITATEDFPFQKHILLFNISIPRHEQITRAELRLYVSCQNHVDPSHDLKGSVVIYDVLDGTDAWDSATETKTFLVSQDIQDEGWETLEVSSAVKRWVRSDSTKSKNKLEVTVESHRKGCDTLDISVPPGSRNLPFFVVFSNDHSSGTKETRLELREMISHEQESVLKKLSKDGSTEAGESSHEEDTDGHVAAGSTLARRKRSAGAGSHCQKTSLRVNFEDIGWDSWIIAPKEYEAYECKGGCFFPLADDVTPTKHAIVQTLVHLKFPTKVGKACCVPTKLSPISVLYKDDMGVPTLKYHYEGMSVAECGCR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Horse
89
Sheep
89
Pig
78
Dog
78
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.

翻译修饰:

A reversible disulfide bond can be formed between the two subunits in the homodimer; this has no effect on GDF2 activity.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in blood plasma (at protein level).

亚基结构:

Homodimer; disulfide-linked. Detected in extracellular fluid as mature homodimer, and in complex with its propeptide. Interacts with ACVRL1, BMPR2 and ACVR2B with high affinity (in vitro). Identified in a complex with ACVRL1 and ACVR2B. Has ten times lower affinity for ACVR2A (in vitro). Interacts with ENG, forming a heterotetramer with a 2:2 stoichiometry. Can form a heteromeric complex with ENG and ACVRL1.

蛋白家族:

Belongs to the TGF-beta family.

文献引用

1). ITE promotes hypoxia-induced transdifferentiation of human pulmonary arterial endothelial cells possibly by activating transforming growth factor-β/Smads and MAPK/ERK pathways. JOURNAL OF CELLULAR BIOCHEMISTRY, 2019 (PubMed: 31297875) [IF=3.0]

Application: WB    Species: human    Sample: HPAECs

FIGURE 6 |Effects of ITE on the expression of TGF‐β1, BMP2 and BMP9 in HPAECs induced by hypoxia. HPAECs were cultured under the conditions of normoxia, normoxia + ITE, hypoxia and hypoxia + ITE, respectively. *P < 0.05, **P < 0.01, and ***P < 0.001. BMP, bone morphogenetic protein; HPAEC, human pulmonary arterial endothelial cell; ITE, 2‐(1′H‐indole‐3′‐carbonyl)‐thiazole‐4‐carboxylicacid methyl ester; TGF‐β, transforming growth factor‐β

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