MRRF Antibody - #AF0770

(3)  
产品: MRRF 抗体
货号: AF0770
描述: Rabbit polyclonal antibody to MRRF
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量: 28kDa; 29kD(Calculated).
蛋白号: Q96E11
RRID: AB_2834403

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(92%)
克隆:
Polyclonal
特异性:
MRRF Antibody detects endogenous levels of total MRRF.
RRID:
AB_2834403
引用格式: Affinity Biosciences Cat# AF0770, RRID:AB_2834403.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2400002D02Rik; MGC93722; mitochondrial; mitochondrial ribosome recycling factor; MRFF; Mrrf; MTRRF; OTTHUMP00000022037; OTTMUSP00000013027; Ribosome recycling factor, mitochondrial; Ribosome recycling factor, mitochondrial precursor; Ribosome-recycling factor; Ribosome-releasing factor; Ribosome-releasing factor, mitochondrial; RP23-324A16.1; RRF; RRFM_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q96E11(RRFM_HUMAN) >>Visit HPA database.

基因/基因ID:
MRRF(92399)
描述:
MRRF Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another. Belongs to the RRF family. 7 isoforms of the human protein are produced by alternative splicing.
序列:
MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
92
Xenopus
67
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q96E11 作为底物

Site PTM Type Enzyme
S112 Phosphorylation
S115 Phosphorylation
T225 Phosphorylation
T245 Phosphorylation

研究背景

功能:

Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity).

细胞定位:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the RRF family.

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