(15)
(11)
产品描述
来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Nestin Antibody detects endogenous levels of total Nestin.
RRID:
AB_2841220
引用格式: Affinity Biosciences Cat# DF7754, RRID:AB_2841220.
引用格式: Affinity Biosciences Cat# DF7754, RRID:AB_2841220.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST_HUMAN; Nestin;
抗原和靶标
免疫原:
A synthesized peptide derived from human Nestin, corresponding to a region within C-terminal amino acids.
基因/基因ID:
文献引用
1). Biomimetic Multichannel Silk Nerve Conduits With Multicellular Spatiotemporal Distributions for Spinal Cord Injury Repair. Advanced materials (Deerfield Beach, Fla.), 2024
(PubMed: 39268784)
[IF=27.4]
2). An anti-inflammatory and neuroprotective biomimetic nanoplatform for repairing spinal cord injury. Bioactive Materials, 2022
(PubMed: 35845318)
[IF=18.9]
3). Hair follicle-MSC-derived small extracellular vesicles as a novel remedy for acute pancreatitis. JOURNAL OF CONTROLLED RELEASE, 2022
(PubMed: 36402231)
[IF=10.5]
4). Neurogenesis promoted by the CD200/CD200R signaling pathway following treadmill exercise enhances post-stroke functional recovery in rats. BRAIN BEHAVIOR AND IMMUNITY, 2019
(PubMed: 31513876)
[IF=8.8]
Application: IF/ICC Species: rat Sample: astrocytes
Fig. 5.| The expression of CD200 and CD200R was up-regulated induced by treadmill exercise after stroke in rats.The effect of treadmill exercise on the protein levels of CD200 and CD200R in the ischemic cortex (A-C) and hippocampus (D-F) at 7 and 28 days after tMCAO. CD200R staining shows protein expression at 7 and 28 days after treadmill exercise post-stroke (G). CD200R colocalizes with activated microglia (Iba1+cells, white arrows), not colocalizes with neural stem cells (Nestin+ cells) and astrocytes (GFAP+ cells), Scale bar: 50 μm. N = 4. Results are showed as means ± SEM. # P < 0.05, ## P < 0.01, ### P < 0.001 versus the Sham group. *P < 0.05, ** P < 0.01, *** P < 0.001 versus the tMCAO group.
5). Mesenchymal stem cells overexpressing neuropeptide S promote the recovery of rats with spinal cord injury by activating the PI3K/AKT/GSK3β signaling pathway. Stem cell research & therapy, 2025
(PubMed: 40022159)
[IF=7.5]
Application: IF/ICC Species: Rat Sample:
Fig. 3 Cell culture and identification of stem cells derived from the nasal mucosa of rats. (a) Immunofluorescence for positive markers (CD44, vimentin, Sox2, and Nestin) on the surfaces of EMSCs and NPS-EMSCs. Scale bar = 100 μm. (b) Phase-contrast microscopy image of EMSCs from primary cultures to the third passage and NPS overexpressing EMSCs. Scale bar = 100 μm; Alkaline phosphatase staining indicates that mesenchymal stem cells derived from the nasal mucosa have osteogenic differentiation ability, and Oil Red O staining reveals that EMSCs have adipose differentiation ability. Scale bar = 20 μm. (c) AM/PI double-staining fluorescence images at 1, 3, and 5 days, with green indicating live cells and red indicating dead cells. Scale bar = 100 μm. (d) Scanning electron microscopy image of the hydrogel scaffold demonstrating its porous structure
6). Effects of RO27-3225 on neurogenesis, PDGFRβ+ cells and neuroinflammation after cerebral infarction. International Immunopharmacology, 2020
(PubMed: 32058930)
[IF=4.8]
Application: IF/ICC Species: Human Sample: neonatal neural stem cells
Fig. 1. RO27-3225 promotes stem cell proliferation in the SVZ after cerebral infarction. (A) Immunofluorescence staining of neonatal neural stem cells (Nestin+/
BrdU+) in the SVZ on day 7 after tMCAO. (B) The quantification results showed that the mice belonging to the tMCAO + saline group had more Nestin+/BrdU+ cells
in the SVZ on day 7 after tMCAO than the mice belonging to the sham + saline group, and RO27-3225 strengthened this increase (*p < 0.05 vs. the corresponding
sham group; #p < 0.05 vs. the tMCAO + RO27-3225 group; n = 6/group). The data are shown as the means ± SDs.
7). Repeated Ketamine Anesthesia during the Neonatal Period Impairs Hippocampal Neurogenesis and Long-Term Neurocognitive Function by Inhibiting Mfn2-Mediated Mitochondrial Fusion in Neural Stem Cells. Molecular neurobiology, 2024
(PubMed: 38200350)
[IF=4.6]
8). miR-872-5p/FOXO3a/Wnt signaling feed-forward loop promotes proliferation of endogenous neural stem cells after spinal cord ischemia–reperfusion injury in rats. The FASEB Journal, 2023
(PubMed: 36607643)
[IF=4.4]
9). Protective effect of bone morphogenetic protein-7 induced differentiation of bone marrow mesenchymal stem cells in rat with acute spinal cord injury. Functional & integrative genomics, 2023
(PubMed: 36849554)
[IF=3.9]
10). Astragaloside IV Treats Parkinson's Disease by Regulating the Proliferation and Differentiation of NSCs through the SHH-Nurr1 Pathway. Stem cells international, 2024
(PubMed: 39257865)
[IF=3.8]
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