(17)
(10)
产品描述
来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Nestin Antibody detects endogenous levels of total Nestin.
RRID:
AB_2841220
引用格式: Affinity Biosciences Cat# DF7754, RRID:AB_2841220.
引用格式: Affinity Biosciences Cat# DF7754, RRID:AB_2841220.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST_HUMAN; Nestin;
抗原和靶标
免疫原:
A synthesized peptide derived from human Nestin, corresponding to a region within C-terminal amino acids.
基因/基因ID:
文献引用
1). Biomimetic Multichannel Silk Nerve Conduits With Multicellular Spatiotemporal Distributions for Spinal Cord Injury Repair. Advanced materials (Deerfield Beach, Fla.), 2024
(PubMed: 39268784)
[IF=27.4]
2). An anti-inflammatory and neuroprotective biomimetic nanoplatform for repairing spinal cord injury. Bioactive Materials, 2022
(PubMed: 35845318)
[IF=18.9]
3). Hair follicle-MSC-derived small extracellular vesicles as a novel remedy for acute pancreatitis. JOURNAL OF CONTROLLED RELEASE, 2022
(PubMed: 36402231)
[IF=10.5]
4). Neurogenesis promoted by the CD200/CD200R signaling pathway following treadmill exercise enhances post-stroke functional recovery in rats. BRAIN BEHAVIOR AND IMMUNITY, 2019
(PubMed: 31513876)
[IF=8.8]
Application: IF/ICC Species: rat Sample: astrocytes
Fig. 5.| The expression of CD200 and CD200R was up-regulated induced by treadmill exercise after stroke in rats.The effect of treadmill exercise on the protein levels of CD200 and CD200R in the ischemic cortex (A-C) and hippocampus (D-F) at 7 and 28 days after tMCAO. CD200R staining shows protein expression at 7 and 28 days after treadmill exercise post-stroke (G). CD200R colocalizes with activated microglia (Iba1+cells, white arrows), not colocalizes with neural stem cells (Nestin+ cells) and astrocytes (GFAP+ cells), Scale bar: 50 μm. N = 4. Results are showed as means ± SEM. # P < 0.05, ## P < 0.01, ### P < 0.001 versus the Sham group. *P < 0.05, ** P < 0.01, *** P < 0.001 versus the tMCAO group.
5). Mesenchymal stem cells overexpressing neuropeptide S promote the recovery of rats with spinal cord injury by activating the PI3K/AKT/GSK3β signaling pathway. Stem cell research & therapy, 2025
(PubMed: 40022159)
[IF=7.5]
6). Neuron-glioma synaptic transmission amplified by free 19S proteasome-mediated AMPAR deubiquitination promotes tumor progression. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 2026
(PubMed: 41667338)
[IF=5.6]
7). Sohlh1 Modulates the Stemness and Differentiation of Glioma Stem-Like Cells by Inactivation of Wnt/β-Catenin Signalling Pathway via SFRP1. Journal of cellular and molecular medicine, 2025
(PubMed: 40418209)
[IF=5.3]
8). Effects of RO27-3225 on neurogenesis, PDGFRβ+ cells and neuroinflammation after cerebral infarction. International Immunopharmacology, 2020
(PubMed: 32058930)
[IF=4.8]
Application: IF/ICC Species: Human Sample: neonatal neural stem cells
Fig. 1. RO27-3225 promotes stem cell proliferation in the SVZ after cerebral infarction. (A) Immunofluorescence staining of neonatal neural stem cells (Nestin+/
BrdU+) in the SVZ on day 7 after tMCAO. (B) The quantification results showed that the mice belonging to the tMCAO + saline group had more Nestin+/BrdU+ cells
in the SVZ on day 7 after tMCAO than the mice belonging to the sham + saline group, and RO27-3225 strengthened this increase (*p < 0.05 vs. the corresponding
sham group; #p < 0.05 vs. the tMCAO + RO27-3225 group; n = 6/group). The data are shown as the means ± SDs.
9). Repeated Ketamine Anesthesia during the Neonatal Period Impairs Hippocampal Neurogenesis and Long-Term Neurocognitive Function by Inhibiting Mfn2-Mediated Mitochondrial Fusion in Neural Stem Cells. Molecular neurobiology, 2024
(PubMed: 38200350)
[IF=4.6]
10). miR-872-5p/FOXO3a/Wnt signaling feed-forward loop promotes proliferation of endogenous neural stem cells after spinal cord ischemia–reperfusion injury in rats. The FASEB Journal, 2023
(PubMed: 36607643)
[IF=4.4]
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