Shh Antibody - #DF7747

>>买2送1 已结束>>
>>赠JD卡 已结束>>
(8)   (4)  
产品: Shh 抗体
货号: DF7747
描述: Rabbit polyclonal antibody to Shh
应用: WB
文献验证: WB
反应: Human, Mouse
预测: Pig, Bovine, Sheep, Rabbit, Dog
蛋白号: Q15465
RRID: AB_2841213

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
Shh Antibody detects endogenous levels of total Shh.
RRID:
AB_2841213
引用格式: Affinity Biosciences Cat# DF7747, RRID:AB_2841213.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

HHG 1; HHG-1; HHG1; HLP 3; HLP3; Holoprosencephaly 3; HPE 3; HPE3; MCOPCB5; shh; SHH_HUMAN; SMMC I; SMMCI; Sonic Hedgehog (Drosophila) homolog; sonic hedgehog homolog (Drosophila); Sonic hedgehog homolog; Sonic hedgehog protein; Sonic hedgehog protein C-product; TPT; TPTPS;

抗原和靶标

免疫原:

A synthesized peptide derived from human Shh, corresponding to a region within the internal amino acids.

基因/基因ID:
SHH(6469)

研究领域

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). Effects of metformin on Sonic hedgehog subgroup medulloblastoma progression: In vitro and in vivo studies. Frontiers in Pharmacology, 2022 (PubMed: 36278239) [IF=5.6]
2). Effects of sulforaphane on prostate cancer stem cells-like properties: In vitro and molecular docking studies. Archives of biochemistry and biophysics, 2024 (PubMed: 39549984) [IF=3.8]
3). Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. DRUG DEVELOPMENT RESEARCH, 2019 (PubMed: 31403228) [IF=3.5]

Application: WB    Species: human    Sample: HIBECs

FIGURE 5 |Curcumin suppresses the TGF-β1-activated Hedgehog signaling in HIBECs. (a) Quantitative analysis of Shh, Gli1, Smo, Ptch-1, and Sufu protein expression.
4). Dihydrotanshinone I Inhibits Pancreatic Cancer Progression via Hedgehog/ Gli Signal Pathway. Current cancer drug targets, 2023 (PubMed: 37018533) [IF=2.3]
5). miRNA-182-5p promotes human bladder cancer proliferation and migration through the FOXF2/SHH axis. Neoplasma, 2022 (PubMed: 35068163) [IF=2.0]
6). Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer. Translational cancer research, 2023 (PubMed: 37969369) [IF=1.5]

Application: WB    Species: Mouse    Sample: liver cancer cell

Figure 2 Ubenimex blocked Hh signaling by targeting CD13 in liver cancer cell lines. (A-C) Western blot analysis of the protein expression levels of CD13, PTCH1, GLI1, SMO, and SHH in Huh7, HepG2, and PLC/PRF/5 cells treated with 0.05 or 0.1 mg/mL ubenimex. Densitometry analysis was used to normalize the expression levels of the proteins to those of β-actin; mean ± SD is indicative of three independent experiments (*, P
7). Inhibitory Effects of Rhein on Renal Interstitial Fibrosis via the SHH-Gli1 Signal Pathway. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35966731)

Application: WB    Species: Rat    Sample:

Figure 3 Inhibitory effect of rhein on SHH signaling pathway in UUO rats. Rhein group rats were administered rhein 2 mg·kg−1·d−1 by oral gavage for 14-d sham group, and UUO group rats were administered equal volumes of water. SHH, Gli1, and Snail protein and mRNA expression levels were determined by western blotting and RT-PCR. Data are presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.
8). Shen-Shuai-Ling Formulation Attenuates Renal Interstitial Fibrosis in Chronic Kidney Disease by Regulating SHH-Gli1 Signaling Pathway. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35190746)

Application: WB    Species: Rat    Sample: renal tissues

Figure 6 Levels of α-SMA, Col I SHH, Gli1, and snail1 in renal tissues in each group. (a) Levels of α-SMA, Col I SHH, Gli1, and snail1 were measured by Western blot. (b) Quantitative analysis. (c) Levels of α-SMA, Col I SHH, Gli1, and snail1 were measured by qPCR. Data were expressed as mean ± SEM. ∗p < 0.05 compared with the Sham group, #p < 0.05 compared with the Model group, △p < 0.05 compared with the CPN group, ☆p < 0.05 compared with the S + C group, ▲p > 0.05 compared with the CPN group, and ★p > 0.05 compared with the C + S group.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.