PINK1 Antibody - #DF7742

Figure 9 TRPM2-mediated Ca2+ influx is involved in the inhibition of the AKT-mTOR pathway in response to cisplatin. Intracellular Ca2+ signals indicated by the fluorescence of Fluo-4 AM in cisplatin (CP, 20 μM) or normal saline (NS) treated Trpm2-/- and WT MEFs (A, B) and mRTECs (C, D) (n = 7). Scale bars, 20 μm and 10μm, respectively. (E) Immunoblotting analysis and quantification of LC3B II in WT MEFs after incubation with clotrimazole (CLT, 10 μM) or vehicle (Veh) for 1 h followed by treatment with CP at the indicated concentration for 24 h. (F) Immunoblotting analysis and quantification of LC3B II in WT MEFs after incubation with 5 μM BAPTA-AM for 1 h followed by treatment with 20 μM CP for 24 h. (G) Immunoblotting analysis and quantification of p-AKT, AKT and p-p70S6K in WT MEFs after incubation with 5 μM BAPTA-AM or 10 μM clotrimazole for 1 h followed by treatment with 20 μM CP for 24 h. (H, I) Mitochondrial Ca2+ signals indicated by the fluorescence of Rhod-2 in mRTECs treated by CP or NS (n = 7). Scale bars, 10 μm. (J) Immunoblotting analysis and quantification of BNIP3 and PINK1 in mRTECs treated by CP or NS. Data are presented as mean±SEM. Statistical analysis was performed using one-way ANOVA with Tukey post-hoc test. ns, not significant; *p < 0.05, **p < 0.01, ***p < 0.001.

Fig. 4 VEGFR3 improves abnormal mitophagy by enhancing PARKIN mitochondrial translocation. A-C The expression levels of SQSTM1 and LC3 II/LC3 I in mitochondrial lysates were measured after stimulation with 10−6 M Ang II for the indicated time in HK-2 cells. Relative protein levels were calculated as fold changes vs. 0 h group. D, E HK-2 cells overexpressing VEGFR3 were incubated with 10−6 M Ang II for 48 h. Representative immunoblots of SQSTM1 and LC3 II/LC3 I in mitochondria and quantification of blot intensities. F TEM was performed to assess mitochondrial damage in HK-2 cells overexpressing VEGFR3 or Vector, stimulation with or without Ang II. Scale bar, 2 μm. G, H Mitotracker Red labeled mitochondria and Lysotracker Green labeled lysosome. Representative confocal microscopy images of lysosome and mitochondria in HK2 cells and summarized data. Scale bar, 20 μm. I and K Immunoblotting analysis and quantitative results of BNIP3, FUNDC1, PINK1 and PARKIN in mitochondrial lysates of HK-2 cells primed with or without MAZ51, followed by Ang II treatment. J and L TOMM20 (green) labeled mitochondria. The colocalization with PARKIN (red) and mitochondria was monitored by confocal microscopy and summarized data. Scale bar, 20 μm. Data were from three or more independent experiments and were presented as the mean ± SEM. Data in (A) through (L) were analyzed with one-way ANOVA. ns, not significant, *P 

Fig. 2.| Effects of PS-MPS on mitochondrial membrane protein and membrane potential in control group and PS-MPS group after 24 h. (A) Relative transcription of mRNA of PINK1; (B) Relative protein expression of PINK1