CD105 Antibody - #DF7735

Fig. 5 Culture, characterization, and transfection of cDPSCs and proliferation of cDPSCs in PuraMatrix. a Cell colonies stained with crystal violet. b, c Verification of osteogenic, adipogenic, and neurogenic differentiation capabilities of cDPSCs. Scale bar of left and right images, 100 μm; scale bar of middle images, 50 μm. *p < 0.05 and **p < 0.01. d Stem cell markers of cDPSCs. Scale bar = 1 mm. e, f Verification of green fluorescence expression and ephrinB2 upregulation in transfected cDPSCs. Scale bar = 100 μm. **p < 0.01. g Alizarin Red S staining of cDPSCs, Vector-cDPSCs (ephrinB2 overexpression control), and EfnB2-cDPSCs (ephrinB2 overexpression) on day 24 of osteogenesis. h Alizarin Red S staining intensity was quantified with ImageJ. *p < 0.05. i Proliferation of cDPSCs (1 × 106 cells/ml) in 0.5%, 0.25%, and 0.125% PuraMatrix. *p < 0.05 and **p < 0.01 vs. 0.25% PuraMatrix; # p < 0.05 and ## p < 0.01 vs. 0.125% PuraMatrix. j Proliferation of cDPSCs at different cell densities (0.25, 0.5, 1, 2, or 4 × 106 cells/ml) in 0.25% PuraMatrix. Data are shown as mean ± SD. Assays were repeated three times

Fig. 5 | Culture, characterization, and transfection of cDPSCs and proliferation of cDPSCs in PuraMatrix. a Cell colonies stained with crystal violet. b,c Verification of osteogenic, adipogenic, and neurogenic differentiation capabilities of cDPSCs. Scale bar of left and right images, 100 μm; scale bar of middle images, 50 μm. *p < 0.05 and **p < 0.01. d Stem cell markers of cDPSCs. Scale bar = 1 mm.

Figure 1. Characterization of MSCs and transduction of MSC-Shh. Immunofluorescence indicated that MSCs exhibited a characteristic immunophenotype of high expressed CD44. The Shh gene expression was observed by measuring the tagged protein GFP with immunofluorescence (A). The Western blot assay showed that the protein level of Shh increased significantly in MSC-Shh compared with the control of MSC-Gfp (B). The Western blot assay showed the expression of CD73 and CD105 of MSC-Shh and the control MSC-Gfp (C). The morphological characteristics of MSC-Shh and MSC-Gfp was assessed (D). MSCs: Mesenchymal stem cells. MSC-Shh: Shh-modified MSCs; MSC-Gfp: Green fluorescent protein-modified MSCs.