FATP1 Antibody - #DF7716

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产品: FATP1 抗体
货号: DF7716
描述: Rabbit polyclonal antibody to FATP1
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Dog, Chicken
分子量: 65 kDa; 71kD(Calculated).
蛋白号: Q6PCB7
RRID: AB_2841185

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(92%), Sheep(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
FATP1 Antibody detects endogenous levels of total FATP1.
RRID:
AB_2841185
引用格式: Affinity Biosciences Cat# DF7716, RRID:AB_2841185.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACSVL5; FATP 1; FATP; FATP1; Fatty acid transport protein 1; FLJ00336; Long chain fatty acid transport protein 1; MGC71751; SLC27A1; Solute carrier family 27 (fatty acid transporter) member 1; Solute carrier family 27 member 1;

抗原和靶标

免疫原:
Uniprot:

Q6PCB7(S27A1_HUMAN) >>Visit HPA database.

基因/基因ID:
SLC27A1(376497)
表达:
Q6PCB7 S27A1_HUMAN:

Highest levels of expression are detected in muscle and adipose tissue small, intermediate levels in small intestine, and barely detectable in liver.

序列:
MRAPGAGAASVVSLALLWLLGLPWTWSAAAALGVYVGSGGWRFLRIVCKTARRDLFGLSVLIRVRLELRRHQRAGHTIPRIFQAVVQRQPERLALVDAGTGECWTFAQLDAYSNAVANLFRQLGFAPGDVVAIFLEGRPEFVGLWLGLAKAGMEAALLNVNLRREPLAFCLGTSGAKALIFGGEMVAAVAEVSGHLGKSLIKFCSGDLGPEGILPDTHLLDPLLKEASTAPLAQIPSKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRMAAFGHHAYRMQAADVLYDCLPLYHSAGNIIGVGQCLIYGLTVVLRKKFSASRFWDDCIKYNCTVVQYIGEICRYLLKQPVREAERRHRVRLAVGNGLRPAIWEEFTERFGVRQIGEFYGATECNCSIANMDGKVGSCGFNSRILPHVYPIRLVKVNEDTMELLRDAQGLCIPCQAGEPGLLVGQINQQDPLRRFDGYVSESATSKKIAHSVFSKGDSAYLSGDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEGVLSRLLGQTDVAVYGVAVPGVEGKAGMAAVADPHSLLDPNAIYQELQKVLAPYARPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGHYLPLNEAVYTRICSGAFAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Chicken
100
Horse
92
Pig
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q6PCB7 作为底物

Site PTM Type Enzyme
K202 Ubiquitination
K225 Ubiquitination
K238 Acetylation
Y246 Phosphorylation
Y248 Phosphorylation
Y266 Phosphorylation
Y267 Phosphorylation
S468 Phosphorylation
K474 Ubiquitination
K475 Ubiquitination
Y501 Phosphorylation

研究背景

功能:

Mediates the ATP-dependent import of long-chain fatty acids (LCFA) into the cell by mediating their translocation at the plasma membrane. Has also an acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism (By similarity).

细胞定位:

Cell membrane>Single-pass membrane protein. Endomembrane system>Single-pass membrane protein. Cytoplasm.
Note: Plasma membrane and intracellular membranes, at least in adipocytes. In adipocytes, but not myocytes, insulin via the mTORC1 signaling pathway induces a rapid translocation of SLC27A1 from intracellular compartments to the plasma membrane, paralleled by increased LCFA uptake. Insulin-dependent translocation from the cytoplasm to the cell membrane is regulated by EPRS1. Predominantly cytoplasmic in myocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest levels of expression are detected in muscle and adipose tissue small, intermediate levels in small intestine, and barely detectable in liver.

亚基结构:

Self-associates. May function as a homodimer (By similarity). Interacts with EPRS1; mediates the translocation of SLC27A1 from the cytoplasm to the plasma membrane thereby increasing the uptake of long-chain fatty acids.

蛋白家族:

Belongs to the ATP-dependent AMP-binding enzyme family.

研究领域

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

文献引用

1). Downregulations of placental fatty acid transporters during cadmium-induced fetal growth restriction. Toxicology (PubMed: 31152847) [IF=4.5]

Application: WB    Species: mouse    Sample: placenta

Fig. 4. |Effects of maternal Cd exposure on the protein expression of placental FATP1, FATP6 and FABP3. (A): FATP1, FATP6 and FABP3 at E16.55; (B): FATP1, FATP6 and FABP3 at E19.5. Each sample group composed of 5 mice. For each mouse, one placenta was taken for protein analysis. The number of samples in each groupfor western blotting assay was 5. E: embryonic day. Results were presented as mean ± SD. *:p < 0.05; compared with the controls.
2). Severe hypoglycemia exacerbates myocardial dysfunction and metabolic remodeling in diabetic mice. MOLECULAR AND CELLULAR ENDOCRINOLOGY (PubMed: 31887336) [IF=4.1]

Application: WB    Species: mouse    Sample: myocardial

Fig. 6. |SH inhibited myocardial metabolism in diabetic mice. Real-time PCR analysis of the gene expression involved in myocardial fatty acid transportation,oxidation, and glucose uptake (n = 8 per group). (B) Gene expression of myocardial transcriptional regulator PPARs (n = 8 per group). (C) Representative immunoblot images of relative protein expression (n = 6 per group).

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