产品: FOXJ3 抗体
货号: AF0602
描述: Rabbit polyclonal antibody to FOXJ3
应用: WB IHC
反应: Human, Mouse
预测: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 68kDa; 69kD(Calculated).
蛋白号: Q9UPW0
RRID: AB_2834393

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
FOXJ3 Antibody detects endogenous levels of total FOXJ3.
RRID:
AB_2834393
引用格式: Affinity Biosciences Cat# AF0602, RRID:AB_2834393.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Forkhead box J3; Forkhead box protein J3; FOXJ3; FOXJ3_HUMAN; KIAA1041; MGC165036; MGC176686;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
FOXJ3 2 isoforms of the human protein are produced by alternative splicing.
序列:
MGLYGQACPSVTSLRMTSELESSLTSMDWLPQLTMRAAIQKSDATQNAHGTGISKKNALLDPNTTLDQEEVQQHKDGKPPYSYASLITFAINSSPKKKMTLSEIYQWICDNFPYYREAGSGWKNSIRHNLSLNKCFLKVPRSKDDPGKGSYWAIDTNPKEDVLPTRPKKRARSVERASTPYSIDSDSLGMECIISGSASPTLAINTVTNKVTLYNTDQDGSDSPRSSLNNSLSDQSLASVNLNSVGSVHSYTPVTSHPESVSQSLTPQQQPQYNLPERDKQLLFSEYNFEDLSASFRSLYKSVFEQSLSQQGLMNIPSESSQQSHTSCTYQHSPSSTVSTHPHSNQSSLSNSHGSGLNTTGSNSVAQVSLSHPQMHTQPSPHPPHRPHGLPQHPQRSPHPAPHPQQHSQLQSPHPQHPSPHQHIQHHPNHQHQTLTHQAPPPPQQVSCNSGVSNDWYATLDMLKESCRIASSVNWSDVDLSQFQGLMESMRQADLKNWSLDQVQFADLCSSLNQFFTQTGLIHSQSNVQQNVCHGAMHPTKPSQHIGTGNLYIDSRQNLPPSVMPPPGYPHIPQALSTPGTTMAGHHRAMNQQHMMPSQAFQMRRSLPPDDIQDDFDWDSIV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Bovine
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UPW0 作为底物

Site PTM Type Enzyme
S22 Phosphorylation
K41 Sumoylation
K41 Ubiquitination
T45 Phosphorylation
T51 Phosphorylation
K55 Ubiquitination
K56 Ubiquitination
T64 Phosphorylation
T65 Phosphorylation
Y81 Phosphorylation
S131 Phosphorylation
K148 Ubiquitination
Y214 Phosphorylation
T216 Phosphorylation
S221 Phosphorylation
S223 Phosphorylation
K280 Ubiquitination
S295 Phosphorylation
S472 Phosphorylation
S489 Phosphorylation
S606 Phosphorylation

研究背景

功能:

Transcriptional activator of MEF2C involved in the regulation pf adult muscle fiber type identity and skeletal muscle regeneration.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

文献引用

1). Multiple roles of FOXJ3 in spermatogenesis: A lesson from Foxj3 conditional knockout mouse models. MOLECULAR REPRODUCTION AND DEVELOPMENT (PubMed: 27739607) [IF=2.5]

Application: WB    Species: mouse    Sample:

Figure 1. Loss of FOXJ3 in different germ cell population. A: FOXJ3 protein abundance in testes at Embryonic Day 15.5 (E15.5), Postnatal Day 3 (PND3), and 1, 2, and 3 weeks old (W). GAPDH served as a loading control. B: FOXJ3 immunostaining of adult mouse testes. Scale bars, 100 μm. C: Strategy for Mvh- or Stra8-driven Cre recombinase-induced deletion of Foxj3 (exon 3) in male germ cells. Black, numbered boxes represent exons in the Foxj3 gene. D: FOXJ3 immunoblot for Mvh-cKO and control testes at PND3. E: FOXJ3 immunoblot for Stra8-cKO and control spermatocyte lysate. GAPDH served as a loading control.

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