Caspase 4/11 Antibody - #DF7609

"FIGURE 6 XBJ reduced the expression of caspase-11 and GSDMD in septic mice. The lung tissues were collected at 24 h after CLP. (A–C). The expressions of GSDMD and caspase-11 were determined with Western blot (A) and quantified results (B,C), respectively (n ≥ 3) (D–F). The neutrophils in lung tissues were isolated and purified at 24 h after CLP. The expressions of caspase-11 and GSDMD were detected by immunofluorescent assay as described in the methods (D). the representative images of immunofluorescent assays (E,F). The quantification of caspase-11 (E) and GSDMD (F) expressions in neutrophils isolated from lung tissues. N > 3/group. The values were indicated as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared with the CLP group."

Fig. 2. Isoflurance pretreatement can attenuate liver injury by reducing noncanonical pyroptosis in liver macrophage A Caspase-11 expression in liver tissues was detected by Western biotting. B. Serum levels of IL-1β and IL-18 were measured. All data are shown as the mean ± SD n (sham) = 5 mice per group, n(IRI) = 5 mice per group *indicates statistical difference at P < 0.05.

Fig. 3. Isoflurance pretreatement can attenuate liver injury by reducing noncanonical pyroptosis in liver macrophage A Representative fluorescence image of caspase-11 expression from liver sections(200x). B. Serum levels of IL-1β and IL-18 were measured. All data are shown as the mean ± SD n (sham) = 5 mice per group, n(IRI) = 5 mice per group *indicates statistical difference at P < 0.05.

FIGURE 2 Olanzapine treatment activated NLRP3/caspase-1 signaling, and increased NLRC4, NLRP6, and IL-1β protein expression in cultured astrocytes. Cultured cells were treated with 0.1, 1, or 10 μM olanzapine for 24 h. (A–E) Western blot was used to evaluate the expression of NLRP3, caspase-1 (20 kDa), GSDMD (53 and 35 kDa), NLRC4, NLRP6, and IL-1β. The data are presented as the mean ± standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control (one-way analysis of variance and post hoc Dunnett’s multiple comparison test; n = 3–4 cultures/western blot group). Con, control; OLZ, olanzapine.