EPN2 Antibody - #AF0682

(2)  
产品: EPN2 抗体
货号: AF0682
描述: Rabbit polyclonal antibody to EPN2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 68kDa; 68kD(Calculated).
蛋白号: O95208
RRID: AB_2834390

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(83%), Bovine(83%), Sheep(83%), Rabbit(92%), Dog(91%)
克隆:
Polyclonal
特异性:
EPN2 Antibody detects endogenous levels of total EPN2.
RRID:
AB_2834390
引用格式: Affinity Biosciences Cat# AF0682, RRID:AB_2834390.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EHB21; EPN 2; Epn2; EPN2_HUMAN; EPS 15 interacting protein 2; EPS-15-interacting protein 2; Eps15 binding protein; Epsin 2; Epsin-2; Epsin2; KIAA1065; OTTHUMP00000065808; OTTHUMP00000065809; OTTHUMP00000065810; OTTHUMP00000065811; OTTHUMP00000065889;

抗原和靶标

免疫原:
Uniprot:

O95208(EPN2_HUMAN) >>Visit HPA database.

基因/基因ID:
EPN2(22905)
表达:
O95208 EPN2_HUMAN:

Highest expression is found in brain. Detected at lower levels in lung and liver.

描述:
This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
序列:
MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKAERAQALKTKERMAQVATGMGSNQITFGRGSSQPNLSTSHSEQEYGKAGGSPASYHGSPEASLCPQHRTGAPLGQSEELQPLSQRHPFLPHLGLASRPNGDWSQPCLTCDRAARATSPRVSSELEQARPQTSGEEELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATVQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSARKTPESFLGPNAALVNLDSLVTRPAPPAQSLNPFLAPGAPATSAPVNPFQVNQPQPLTLNQLRGSPVLGTSTSFGPGPGVESMAVASMTSAAPQPALGATGSSLTPLGPAMMNMVGSVGIPPSAAQATGTTNPFLL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
92
Dog
91
Pig
83
Bovine
83
Sheep
83
Horse
75
Chicken
67
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95208 作为底物

Site PTM Type Enzyme
T2 Phosphorylation
S4 Phosphorylation
S5 Phosphorylation
K11 Ubiquitination
K69 Ubiquitination
Y83 Phosphorylation
K107 Ubiquitination
K117 Ubiquitination
K128 Ubiquitination
K135 Ubiquitination
K141 Ubiquitination
T150 Phosphorylation
S163 Phosphorylation
R170 Methylation
S172 Phosphorylation
S173 Phosphorylation
S178 Phosphorylation
S182 Phosphorylation
Y186 Phosphorylation
S192 Phosphorylation
S195 Phosphorylation
S199 Phosphorylation
S237 Phosphorylation
T249 Phosphorylation
T272 Phosphorylation
S273 Phosphorylation
S287 Phosphorylation
T316 Phosphorylation
K318 Ubiquitination
S327 Phosphorylation
S408 Phosphorylation
S420 Phosphorylation
K423 Acetylation
K423 Methylation
K423 Ubiquitination
S426 Phosphorylation
S486 Phosphorylation
S492 Phosphorylation
S504 Phosphorylation
T508 Phosphorylation
S570 Phosphorylation
S622 Phosphorylation
S628 Phosphorylation

研究背景

功能:

Plays a role in the formation of clathrin-coated invaginations and endocytosis.

翻译修饰:

Ubiquitinated.

细胞定位:

Cytoplasm. Cytoplasmic vesicle>Clathrin-coated vesicle.
Note: In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest expression is found in brain. Detected at lower levels in lung and liver.

亚基结构:

Binds EPS15 (By similarity). Interacts with ITSN1 (By similarity). Binds AP-2 and clathrin. Interacts with UBQLN2.

蛋白家族:

The NPF repeat domain is involved in EPS15 binding.

The DPW repeat domain is involved in AP-2 and clathrin binding.

Belongs to the epsin family.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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