产品: Perilipin-1 抗体
货号: DF7602
描述: Rabbit polyclonal antibody to Perilipin-1
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
蛋白号: O60240
RRID: AB_2841093

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Perilipin-1 Antibody detects endogenous levels of total Perilipin-1.
RRID:
AB_2841093
引用格式: Affinity Biosciences Cat# DF7602, RRID:AB_2841093.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin; Perilipin-1; PerilipinA; PLIN; PLIN1; PLIN1_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Perilipin-1, corresponding to a region within C-terminal amino acids.

基因/基因ID:

研究领域

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

文献引用

1). Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity. Food Chemistry, 2022 (PubMed: 35749873) [IF=8.5]

2). Promotion effect of salt on intramuscular neutral lipid hydrolysis during dry-salting process of porcine (biceps femoris) muscles by inducing phosphorylation of ATGL, HSL and their regulatory proteins of Perilipin1, ABHD5 and G0S2. Food chemistry, 2022 (PubMed: 34815115) [IF=8.5]

3). PLIN1 suppresses glioma progression through regulating lipid metabolism. Cell death & disease, 2025 (PubMed: 39870645) [IF=8.1]

4). miR-133a silencing rescues glucocorticoid-induced bone loss by regulating the MAPK/ERK signaling pathway. Stem Cell Research & Therapy, 2021 (PubMed: 33781345) [IF=7.5]

Application: IF/ICC    Species: rat    Sample: distal femur

Fig. 7 |Silencing miR-133a attenuates marrow fat accumulation and trabecula damage in distal femurs of GIO rats.a Representative images of H&E staining showing trabecula and marrow adipocytes in distal femur of each group. b Quantification of number of adipocytes in distal femur of each group. c, d Representative images of immunofluorescence staining (c) and quantification of perilipin area /total area (T.Ar) in distal femuro the MP group)

5). Crocetin Alleviates Ovariectomy-Induced Metabolic Dysfunction through Regulating Estrogen Receptor β. Journal of agricultural and food chemistry, 2021 (PubMed: 34851635) [IF=5.7]

6). Sex hormone-binding globulin improves lipid metabolism and reduces inflammation in subcutaneous adipose tissue of metabolic syndrome-affected horses. Frontiers in molecular biosciences, 2023 (PubMed: 38146533) [IF=5.0]

Application: WB    Species: Human    Sample: adipose tissue

FIGURE 8 Influence of SHBG treatment of lipid metabolism related modulators interplay in SAT (A–N). The relative expression levels of LPL (A), SCD (B), PNPLA2 (C), PLIN1 (D), PPARA (E), FASN (F) in SAT were analyzed by RT-PCR. Protein levels of LPL (G), PLIN1 (H), FASN (I), SCD1 (J), ATGL (K, L) and HSL (M) were analyzed by Western blot ((N), representative membranes). Representative data are shown as mean ± SD.

7). Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function. JOURNAL OF LIPID RESEARCH, 2019 (PubMed: 30552289) [IF=5.0]

Application: WB    Species: pig    Sample: porcine intramuscular preadipocytes

Fig.?5.|Melatonin-activated PKA and ERK1/2 mediate lipolysis in porcine intramuscular preadipocytes. A–J: Fully differentiated adipocytes were treated with control, 1 mM of melatonin, and 1 mM of melatonin plus 10 M of 4-P-PDOT for 24 h. The expression levels of PKA (A, B), ERK1/2 (C, D), HSL (E, F), PLIN1 (G, H), and ATGL (I, J) and the phosphorylation levels of PKA (p-PKA Thr197) (A, B), ERK1/2 (p-ERK1/2 Thr202/Tyr204) (C, D), HSL (pHSL Ser660) (E, F), PLIN1 (p-PLIN1 Ser522) (G, H), and -tubulin (I, J) were evaluated by Western blotting. The results are represented as the mean ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001; n = 3).

8). Equisetin inhibits adiposity through AMPK-dependent regulation of brown adipocyte differentiation. Heliyon, 2024 (PubMed: 38327434) [IF=4.0]

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