SREBP2 Antibody - #DF7601

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产品: SREBP2 抗体
货号: DF7601
描述: Rabbit polyclonal antibody to SREBP2
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 124kDa; 124kD(Calculated).
蛋白号: Q12772
RRID: AB_2841092

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
SREBP2 Antibody detects endogenous levels of total SREBP2.
RRID:
AB_2841092
引用格式: Affinity Biosciences Cat# DF7601, RRID:AB_2841092.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AI608257; bHLHd2; Class D basic helix-loop-helix protein 2; OTTHUMP00000028740; Processed sterol regulatory element-binding protein 2; SRBP2_HUMAN; SREBF 2; Srebf2; SREBF2 protein; SREBP 2; SREBP-2; SREBP2; SREBP2gc; sterol regulatory element binding factor 2; Sterol regulatory element binding protein 2; Sterol regulatory element binding transcription factor 2; Sterol regulatory element-binding transcription factor 2;

抗原和靶标

免疫原:
Uniprot:

Q12772(SRBP2_HUMAN) >>Visit HPA database.

基因/基因ID:
SREBF2(6721)
表达:
Q12772 SRBP2_HUMAN:

Ubiquitously expressed in adult and fetal tissues.

序列:
MDDSGELGGLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGSGGSGSSSGSSGSSSSSSNGRGSSSGAVDPSVQRSFTQVTLPSFSPSAASPQAPTLQVKVSPTSVPTTPRATPILQPRPQPQPQPQTQLQQQTVMITPTFSTTPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSSGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNEVDLKIEDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTSLLQWGGAHDSDQHPHSGSGRSVLSFESGSGGWFDWMMPTLLLWLVNGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAGNLQTCLAVLGRALPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQCRRATPATEAGFEDEAKTSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTRCGGKLGFLASYFLSRAQSLCGPEHSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSVGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFTKVERIPKALEVTESPLVKAIFHACRAMHASLPGKADGQQSSFCHCERASGHLWSSLNVSGATSDPALNHVVQLLTCDLLLSLRTALWQKQASASQAVGETYHASGAELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTTQSTKHGEVDAWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRRSCNDCQQMIVKLGGGTAIAAS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.

翻译修饰:

At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.

Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.

细胞定位:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed in adult and fetal tissues.

亚基结构:

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminal domain) with RNF139.

蛋白家族:

Belongs to the SREBP family.

文献引用

1). NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes. LIFE SCIENCES, 2020 (PubMed: 32976883) [IF=5.2]

Application: WB    Species: rat    Sample: BRL-3A hepatocytes

Fig. 3. |NPY promotes pSREBP2 expression and accelerates the processing of pSREBP2 to mSREBP2 both in vivo and in vitro.(A) Hepatic portal vein injection of NPY (100 μg/kg) significantly increased both pSREBP2 expression and mSREBP2 maturation after 1 h in rats.
2). Digeda-4 decoction and its disassembled prescriptions improve dyslipidemia and apoptosis by regulating AMPK/SIRT1 pathway on tyloxapol-induced nonalcoholic fatty liver disease in mice. Journal of Ethnopharmacology, 2023 (PubMed: 37348794) [IF=4.8]
3). Alcohol intake exacerbates experimental autoimmune prostatitis through gut microbiota driving cholesterol biosynthesis-mediated Th17 differentiation. International immunopharmacology, 2024 (PubMed: 39029231) [IF=4.8]
4). Resveratrol Improves the Progression of Osteoarthritis by Regulating the SIRT1-FoxO1 Pathway-Mediated Cholesterol Metabolism. MEDIATORS OF INFLAMMATION, 2023 (PubMed: 36643587) [IF=4.4]
5). Hypermethylation of the CTRP9 promoter region promotes Hcy induced VSMC lipid deposition and foam cell formation via negatively regulating ER stress. Scientific reports, 2023 (PubMed: 37945738) [IF=3.8]

Application: WB    Species: Human    Sample: VSMC

Figure 4. Effects of CTRP9 on the expression of ERs marker proteins and lipid homeostasis regulatory factors in VSMC. (A) CTRP9 mRNA and protein expression levels. (B) ATF6a mRNA and protein expression levels. (C) mRNA and protein expression levels of CRP78. (D) Expression levels of IRE1a mRNA and p-IRE1a protein. (E) Expression levels of PERK mRNA and p-PERK protein. (F) SREBP1c mRNA and protein expression levels. (G) SREBP2 mRNA and protein expression levels. The experiment was performed in triplicate, and the representative images are shown. *P 
6). MiR-204-5p promotes apoptosis and inhibits migration of osteosarcoma via targeting EBF2. BIOCHIMIE, 2019 (PubMed: 30529043) [IF=3.3]

Application: WB    Species: human    Sample: MG63 cells

Figure 4.| MiR-204-5p targets EBF2 .D. WB analysis of EBF2 protein levels in MG63 cells transfected with control miRNA (miR-NC), miR-204-5p, control antagomiR (anti-miR-NC) and antagomiR-204-5p (anti-miR-204-5p).

Application: IHC    Species: human    Sample: Migrated Saos-2 and MG63 cells

Figure 5. |EBF2 inhibits apoptosis and promotes migration and invasion of osteosarcoma.E. Migrated Saos-2 and MG63 cells transfected with control siRNA (si-NC) and EBF2 siRNA (si-EBF2). Lower panels are statistics of transwell results. Data were presented as means ± SD. *=P<0.05, **=P<0.01 and ***=P<0.001
7). Sleeve Gastrectomy Suppresses Hepatic De Novo Cholesterogenesis and Improves Hepatic Cholesterol Accumulation in Obese Rats with Type 2 Diabetes Mellitus. NUTRITION, 2022 (PubMed: 34952362) [IF=3.2]
8). The Hypolipidemic Effect of Hawthorn Leaf Flavonoids through Modulating Lipid Metabolism and Gut Microbiota in Hyperlipidemic Rats. Evidence-based complementary and alternative medicine : eCAM, 2021 (PubMed: 36425260)

Application: WB    Species: Rat    Sample:

Figure 14 The potential drug targets for antihyperlipidemia by HLF. (a) The level of CYP7A1 in the intestinal tissue; (b) the level of HMGCR in the plasma level; (c) the expression of related targets in the liver; (d) the expression of related targets in the intestine. These data are presented as the mean ± SD (n = 3).
9). The Hypolipidemic Effect of Dalbergia odorifera T. C. Chen Leaf Extract on Hyperlipidemic Rats and Its Mechanism Investigation Based on Network Pharmacology. Evidence-Based Complementary and Alternative Medicine, 2021 (PubMed: 34987591)

Application: WB    Species: Rat    Sample: liver

Figure 9 Western blotting. Effect of D. odorifera leaves on the protein levels of SCAP, SREBP-2, HMGCR, and LDLR in the liver.

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