Phospho-ULK1 (Ser556) Antibody - #DF7587

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产品: 磷酸化 ULK1 (Ser556) 抗体
货号: DF7587
描述: Rabbit polyclonal antibody to Phospho-ULK1 (Ser556)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: O75385
RRID: AB_2841078

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   规格 价格 库存
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-ULK1 (Ser556) Antibody detects endogenous levels of ULK1 only when phosphorylated at S556.
RRID:
AB_2841078
引用格式: Affinity Biosciences Cat# DF7587, RRID:AB_2841078.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATG 1; ATG1; ATG1 autophagy related 1 homolog; ATG1A; Autophagy related protein 1 homolog; Autophagy-related protein 1 homolog; FLJ38455; FLJ46475; hATG1; KIAA0722; Serine/threonine protein kinase ULK1; Serine/threonine protein kinase Unc51.1; Serine/threonine-protein kinase ULK1; ULK 1; ULK1; ULK1_HUMAN; Unc 51 (C. elegans) like kinase 1; UNC 51; Unc 51 like kinase 1; Unc-51 like kinase 1 (C. elegans); Unc-51-like kinase 1; UNC51; UNC51, C. elegans, homolog of; Unc51.1;

抗原和靶标

免疫原:

A synthesized peptide derived from human ULK1around the phosphorylation site of S556.

基因/基因ID:
ULK1(8408)

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

文献引用

1). Low selenium and T-2 toxin may be involved in the pathogenesis of Kashin-Beck disease by affecting AMPK/mTOR/ULK1 pathway mediated autophagy. Ecotoxicology and environmental safety, 2024 (PubMed: 38810288) [IF=6.2]

Application: WB    Species: Rat    Sample:

Fig. 2. mRNA and proteins expressions of AMPK/mTOR/ULK1 pathway and autophagy markers in chondrocytes from normal control (NC) and Kashin-beck disease (KBD). A. mRNA expression of AMPK, mTOR, ULK1, and ATG13 in NC and KBD chondrocytes. n≥4. B. mRNA expression of LC3B, Beclin1, p62 in NC and KBD chondrocytes. n≥4. C. Proteins expression of p-AMPK, AMPK, p-mTOR, mTOR, p-ULK1, ULK1, p-ATG13, ATG13 in NC and KBD chondrocytes. D. Proteins expression level of autophagy markers (LC3-II/I, Beclin1, p62) in NC and KBD chondrocytes. n=3. The data are shown as mean ± SD for three independent experiments. *Statistically significant difference between NC and KBD (*P
2). Prototheca bovis induces autophagy in bovine mammary epithelial cells via the HIF-1α and AMPKα/ULK1 pathway. Frontiers in Immunology, 2022 (PubMed: 36148236) [IF=5.7]

Application: WB    Species: bovine    Sample: bMECs

Figure 2 P. bovis infection induced autophagy via activated AMPKα/ULK1 signaling in bMECs. (A) and (B) Western blot analyses of AMPKα, p-AMPKα, p-mTOR, ULK1, p-ULK1, SQSTM1/p62, Atg5 and LC3II/LC3I in bMECs. The right panel is protein quantification with ImageJ software. The bMECs were infected with P. bovis and treated with rapamycin (20 µM) for 6 hAdditionally, bMECs were pretreated with 3MA (5 mM) for 2 h, and then infected with P. bovis for 6 h Data represent means ± SD of 3 independent experiments, compared to the control group, *P < 0.05 and **P < 0.01; compared to the P. bovis group
3). Xue-Jie-San prevents the early development of colitis-associated intestinal fibrosis by blocking Notch1 and FGL1 signaling pathways. Journal of ethnopharmacology, 2023 (PubMed: 37263315) [IF=4.8]

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