MCP1 Antibody - #DF7577

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产品: MCP1 抗体
货号: DF7577
描述: Rabbit polyclonal antibody to MCP1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Horse
分子量: 14 kDa; 11kD(Calculated).
蛋白号: P13500
RRID: AB_2841069

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Horse(83%)
克隆:
Polyclonal
特异性:
MCP1 Antibody detects endogenous levels of total MCP1.
RRID:
AB_2841069
引用格式: Affinity Biosciences Cat# DF7577, RRID:AB_2841069.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C-C motif chemokine 2; CCL2; CCL2_HUMAN; Chemokine (C C motif) ligand 2; GDCF 2; GDCF-2; GDCF2; HC11; HSMCR30; JE; MCAF; MCP 1; MCP-1; MCP1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A (Cys Cys), member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF;

抗原和靶标

免疫原:

A synthesized peptide derived from human MCP1, corresponding to a region within the internal amino acids.

Uniprot:

P13500(CCL2_HUMAN) >>Visit HPA database.

基因/基因ID:
CCL2(6347)
表达:
P13500 CCL2_HUMAN:

Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level) (PubMed:23765988). Expressed in monocytes (PubMed:2513477).

序列:
MKVSAALLCLLLIAATFIPQGLAQPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
83
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

翻译修饰:

Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

N-Glycosylated.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Expressed in monocytes.

亚基结构:

Monomer or homodimer; in equilibrium. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.

蛋白家族:

Belongs to the intercrine beta (chemokine CC) family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

文献引用

1). Gut Metabolite Indole-3-Propionic Acid Regulates Macrophage Autophagy Through PPT1 Inhibiting Aging-Related Myocardial Fibrosis. Advanced Science, 2025 [IF=14.1]

Application: IHC    Species: Mouse    Sample:

Figure 5 Transgenic knockout of macrophage PPT1 improves cardiac inflammatory infiltration and myocardial fibrosis in D-gal induce-aged mice. A) Schematic of the experimental design. B) Representative echocardiographic graphs. C–J) Echocardiographic measurements of LVEF, LVFS, LVIDd, LVIDs, LVPWd, LVPWs, IVSd, and IVSs. K) H&E staining of left ventricles. Magnification: 200×, scale bar = 50 µm. L) Masson's staining of left ventricles. Magnification: 200×, scale bar = 50 µm. M,N) Representative images of IHC staining with MCP-1 and α-SMA antibody. Magnification: 200×, scale bar = 50 µm. O) Statistical graphs of Masson's staining. P,Q) Statistical graphs of IHC staining with MCP-1 and α-SMA antibody. R) Representative images of the immunofluorescence of CD68, PPT1, and DAPI in mice heart. Magnification: 200×, scale bar = 20 µm. S) Statistical analysis of COL3A1, COL1A1, PPT1, IL-6, and TNF-α mRNA level. T,U) Representative images and statistical analysis of COL3A1, COL1A1, PPT1, IL-6, and TNF-α at protein level. (n = 5–6, data are expressed as mean ± SEM, **p < 0.01 vs the WT group; #p < 0.05, ##p < 0.01 vs the D-gal group).
2). Outer membrane vesicles derived from gut microbiota mediate tubulointerstitial inflammation: a potential new mechanism for diabetic kidney disease. Theranostics, 2023 (PubMed: 37554279) [IF=12.4]

Application: IHC    Species: Rat    Sample:

Figure 1 Gut microbiota depletion attenuates systemic and tubulointerstitial inflammation in diabetic rats. Gut microbiota of DM rats was depleted by treatment with broad-spectrum antibiotics administered in the drinking water (DM+AB). (A) Serum inflammatory cytokines, including IL-1β, TNF-α, MCP-1 and IL-6, were measured by ELISA (n=4). (B) Tubulointerstitial inflammation was detected by immunohistochemical staining (scale bar, 500 μm and 100 μm, original magnification × 200). (C-D) Expression levels of inflammatory proteins in renal cortex were detected by Western blot analysis and quantified (n=4-5). (E-F) Histopathological injuries of tubulointerstitium were evaluated by PAS staining (scale bar, 500 μm and 100 μm, original magnification × 200) and quantified (n=5).

Application: WB    Species: Rat    Sample:

Figure 1 Gut microbiota depletion attenuates systemic and tubulointerstitial inflammation in diabetic rats. Gut microbiota of DM rats was depleted by treatment with broad-spectrum antibiotics administered in the drinking water (DM+AB). (A) Serum inflammatory cytokines, including IL-1β, TNF-α, MCP-1 and IL-6, were measured by ELISA (n=4). (B) Tubulointerstitial inflammation was detected by immunohistochemical staining (scale bar, 500 μm and 100 μm, original magnification × 200). (C-D) Expression levels of inflammatory proteins in renal cortex were detected by Western blot analysis and quantified (n=4-5). (E-F) Histopathological injuries of tubulointerstitium were evaluated by PAS staining (scale bar, 500 μm and 100 μm, original magnification × 200) and quantified (n=5).
3). Ly6G+ Neutrophils and Interleukin-17 Are Essential in Protection against Rodent Malaria Caused by Plasmodium berghei ANKA. Research (Washington, D.C.), 2024 (PubMed: 39703777) [IF=11.0]
4). HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury. CELLULAR & MOLECULAR BIOLOGY LETTERS, 2021 (PubMed: 34479471) [IF=9.2]

Application: WB    Species: human    Sample: HUVECs

Fig. 3 | Efects of inhibition of HIF-1α on expression of infammation after hyperglycemic and hypoxia.b Cells were treated with KC7F2 (10 µM) or si-HIF-1α for 48 h following combined stimulation, and IL-6, IL-8, ICAM-1, MCP-1, and c HIF-1α levels were analyzed. The relative density of IL-6, IL-8,ICAM-1, and MCP-1 (d) was normalized according to GAPDH expression.

Application: WB    Species: Human    Sample: HUVECs

Fig. 3 Effects of inhibition of HIF-1α on expression of inflammation after hyperglycemic and hypoxia. a Cells were treated with glucose (25 mM) or with combined exposure to high glucose and hypoxia for 6, 12, 24, and 48 h. Exposure to glucose alone or combined stimulation with hypoxia significantly increased gene expression of HIF-1α. b Cells were treated with KC7F2 (10 µM) or si-HIF-1α for 48 h following combined stimulation, and IL-6, IL-8, ICAM-1, MCP-1, and c HIF-1α levels were analyzed. The relative density of IL-6, IL-8, ICAM-1, and MCP-1 (d) was normalized according to GAPDH expression. Importantly, the downregulation of HIF-1α decreased the secretion of IL-6, IL-8, ICAM-1 and MCP-1 based on ELISA and qRT-PCR assays (e–f). n = 3; *p < 0.05 and **p < 0.01 vs. DMSO. DMSO-treated cells, DMSO; HIF-1α inhibitors KC7F2 (10 µM)-treated cells, KC7F2 (10 µM); si-HIF-1α, small interfering RNA HIF-1α; HG, high glucose; HG + Hypoxia, combined stimulus with high glucose and hypoxia; NG, control
5). Micheliolide Ameliorates Diabetic Kidney Disease by Inhibiting Mtdh-mediated Renal Inflammation in Type 2 Diabetic db/db Mice. PHARMACOLOGICAL RESEARCH, 2019 (PubMed: 31669149) [IF=9.1]
6). A bioactive xyloglucan polysaccharide hydrogel mechanically enhanced by Pluronic F127 micelles for promoting chronic wound healing. International journal of biological macromolecules, 2024 (PubMed: 39047998) [IF=7.7]
7). Pyrogallol enhances therapeutic effect of human umbilical cord mesenchymal stem cells against LPS-mediated inflammation and lung injury via activation of Nrf2/HO-1 signaling. Free radical biology & medicine, 2022 (PubMed: 36028178) [IF=7.1]
8). Acetyl-CoA synthetase 2 promotes diabetic renal tubular injury in mice by rewiring fatty acid metabolism through SIRT1/ChREBP pathway. Acta Pharmacologica Sinica, 2023 (PubMed: 37770579) [IF=6.9]
9). Oxoaporphine Pr(III) complex inhibits hepatocellular carcinoma progression and metastasis by disrupting tumor cell-macrophage crosstalk. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023 (PubMed: 37976890) [IF=6.9]
10). Interruption of TRPC6-NFATC1 signaling inhibits NADPH oxidase 4 and VSMCs phenotypic switch in intracranial aneurysm. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023 (PubMed: 37002575) [IF=6.9]
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