LEF1 Antibody - #DF7570

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产品: LEF1 抗体
货号: DF7570
描述: Rabbit polyclonal antibody to LEF1
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: Q9UJU2
RRID: AB_2841063

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
LEF1 Antibody detects endogenous levels of total LEF1.
RRID:
AB_2841063
引用格式: Affinity Biosciences Cat# DF7570, RRID:AB_2841063.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp586H0919; FLJ46390; LEF 1; LEF-1; Lef1; LEF1_HUMAN; Lymphoid enhancer binding factor 1; Lymphoid enhancer-binding factor 1; T cell specific transcription factor 1 alpha; T cell-specific transcription factor 1-alpha; TCF 1 alpha; TCF1 alpha; TCF1-alpha; TCF10; TCF1alpha; TCF7L3; Transcription factor T cell specific 1 alpha;

抗原和靶标

免疫原:

A synthesized peptide derived from human LEF1, corresponding to a region within the internal amino acids.

基因/基因ID:
LEF1(51176)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Organismal Systems > Endocrine system > Melanogenesis.

文献引用

1). PRICKLE1, a Wnt/PCP signaling component, is overexpressed and associated with inferior prognosis in acute myeloid leukemia. Journal of Translational Medicine, 2021 (PubMed: 34001134) [IF=7.4]

Application: WB    Species: human    Sample:

Fig. 7| High expression of PRICKEL1 accompanied with core PCP pathway components and the migration and invasion components up-regulation in AML patients. a The amounts of the indicated PCP proteins were analyzed by Western blotting in AML patients (n=5) and normal controls(n=3).
2). Liraglutide Regulates Bone Destruction and Exhibits Anti-inflammatory Effects in Periodontitis In vitro and In vivo. JOURNAL OF DENTISTRY, 2020 (PubMed: 32119967) [IF=4.8]

Application: WB    Species: human    Sample: hPDLCs

Fig. 4.| LIRA enhanced the osteogenic differentiation of hPDLCs in an inflammatory microenvironment via partial inhibition of the Wnt/β-catenin signaling pathway.(A) Expression levels of nuclear β-catenin (N-β-cat) and nuclear LEF1 (N-LEF1) in the four groups. *P < 0.05 vs. controls, **P < 0.01 vs. controls, #P < 0.05 vs. The LPS group, ##P < 0.01 vs. the LPS group.
3). miR-140-y targets TCF4 to regulate the Wnt signaling pathway and promote embryonic feather follicle development in Hungarian white goose. Poultry science, 2024 (PubMed: 38350393) [IF=3.8]

Application: WB    Species: goose    Sample: dermal fibroblast cells

Figure 8. miR-140-y overexpression suppresses the expression of Wnt pathway related genes and inhibits the proliferation of dermal fibroblast cells. (A) Relative mRNA expression of Wnt pathway related genes was measured in dermal fibroblast cells 24 h after transfection with NC or miR-140-y mimics by qPCR. (B–C) Relative protein expression of Wnt pathway related genes was measured in dermal fibroblast cells 72 h after transfection with NC or miR-140-y mimics by Western blot. (D–E) Representative observations of the EdU assay of dermal fibroblast cells after transfection with NC and miR-140-y mimics (Bars: 100 µm). Red illustrate the EdU staining and blue display the cell nuclei stained with Hoechst 33342. (F) The mRNA expression of proliferation related gene (PCNA). (G-H) Protein expression level of proliferation related gene (PCNA). The data were shown as mean ± SEM, n = 3, * p < 0.05, ** p < 0.01.
4). Src-IL-18 signaling regulates the secretion of atrial natriuretic factor in hypoxic beating rat atria. Kardiologia Polska, 2021 (PubMed: 34176112) [IF=3.7]

Application: WB    Species: rat    Sample: atria

Figure 4. | A, B. Effect of IL-18 on the protein levels of ATF3, TCF3, TCF4 and LEF1.
5). NOX4 stimulates ANF secretion via activation of the Sirt1/Nrf2/ATF3/4 axis in hypoxic beating rat atria. Molecular Medicine Reports, 2022 (PubMed: 35029280) [IF=3.4]

Application: WB    Species: rat    Sample: atria

Figure 5.| Effect of nuclear factor erythroid‑2‑related factor 2 inhibitor on TCF3, TCF4 and LEF1 protein expression induced by hypoxia in beating rat atria.(A)Representative western blots.

Application: IF/ICC    Species: rat    Sample: atria

Figure 5.| Effect of nuclear factor erythroid‑2‑related factor 2 inhibitor on TCF3, TCF4 and LEF1 protein expression induced by hypoxia in beating rat atria. (C) LEF1 protein expression was detected using immunofluorescence staining (scale bar, 100 µm). Data are presented as the mean ± SE. n=5. ***P<0.001 vs. control; ###P<0.001 vs. hypoxia. TCF, T‑cell factor; LEF1, lymphoid enhancer factor 1; cont, control; hy, hypoxia; ML, ML385.

Application: WB    Species: Rat    Sample:

Figure 5. Effect of nuclear factor erythroid-2-related factor 2 inhibitor on TCF3, TCF4 and LEF1 protein expression induced by hypoxia in beating rat atria. (A) Representative western blots. (B) Quantification of protein expression levels. (C) LEF1 protein expression was detected using immunofluorescence staining (scale bar, 100 µm). Data are presented as the mean ± SE. n=5. ***P<0.001 vs. control; ###P<0.001 vs. hypoxia. TCF, T-cell factor; LEF1, lymphoid enhancer factor 1; cont, control; hy, hypoxia; ML, ML385.

Application: IF/ICC    Species: Rat    Sample:

Figure 5. Effect of nuclear factor erythroid-2-related factor 2 inhibitor on TCF3, TCF4 and LEF1 protein expression induced by hypoxia in beating rat atria. (A) Representative western blots. (B) Quantification of protein expression levels. (C) LEF1 protein expression was detected using immunofluorescence staining (scale bar, 100 µm). Data are presented as the mean ± SE. n=5. ***P<0.001 vs. control; ###P<0.001 vs. hypoxia. TCF, T-cell factor; LEF1, lymphoid enhancer factor 1; cont, control; hy, hypoxia; ML, ML385.
6). PITX2 functions as a transcription factor for GPX4 and protects pancreatic cancer cells from ferroptosis. Experimental cell research, 2024 (PubMed: 38710403) [IF=3.3]
7). Cholecystokinin regulates atrial natriuretic peptide secretion through activation of NOX4-Sirt1-LEF1 signaling in beating rat hypoxic atria. Peptides, 2024 (PubMed: 39326462) [IF=2.8]
8). The WNT/Ca2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria. The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 2022 (PubMed: 36302622) [IF=1.6]

Application: WB    Species: Rat    Sample:

Fig. 9 Effects of phospholipase C (PLC) (A), protein kinase C (PKC) (B) and TAK1 (C) antagonists on Wnt agonist 1-induced lymphoid enhancer factor 1 (LEF1) expression in beating rat atria.

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