GM130 Antibody - #DF7556

Figure 3 Arf1 participated in Golgi apparatus distribution during mouse oocyte meiosis. A) Representative images of Golgi distribution at the GV stage in the control and Arf1‐KD oocytes. Red, Golgi; blue, DNA. Scale bar = 5 µm. B) The rate of abnormal Golgi distribution in GV oocytes significantly increased after Arf1 depletion. C) Representative images of Golgi distribution in the GV stage in the control and Myt1‐KD oocytes. Red, Golgi; blue, DNA. Scale bar = 5 µm. D) Screening vesicle transport–related proteins connected with Arf1 by mass spectrometry analysis. E) Co‐IP results indicated that Arf1 was associated with cis‐Golgi marker GM130, GTPase Rab35, and Miro2, but not Rab1A. F) Western blot analysis showed a significant decrease in the GM130 and Rab35 protein levels in the Arf1‐KD oocytes compared with the control oocytes. Western blot analysis revealed no difference in Miro2 expression between the control and Arf1‐KD groups. Overall, 180 oocytes were used in each group. G) Representative images of GM130 distribution in the GV stage in the control and Arf1‐KD oocytes. The fluorescence intensity of the GM130 signal significantly decreased after Arf1 depletion. Green, GM130; blue, DNA. Scale bar = 20 µm. H) Representative images of Rab35 distribution in the GV stage in the control and Arf1‐KD oocytes. The fluorescence intensity of the Rab35 signal significantly decreased after Arf1 depletion. Red, Rab35; blue, DNA. Scale bar = 20 µm. I) Representative images of Golgi distribution in the MI stage in the control and Arf1‐KD oocytes. Red, Golgi; blue, DNA. Scale bar = 5 µm. J) The rate of abnormal Golgi distribution in MI oocytes significantly increased in the Arf1‐KD group compared with the control group. The data are presented as mean ± SEM from at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Fig. 8 Activating S100B/RAGE signal promotes ADAM17 translocation in RAECs. A Representative confocal images showing the subcellular localization of ADAM17 and GM130 (the Golgi complex-associated protein) in RAECs treated with stretch injured-conditioned medium, exogenous S100B, and injured medium plus S100B inhibitor ONO-2506 or RAGE inhibitor FPS-ZM-1. Scale bar = 20 μm. The immunofluorescence intensity of GM130+/ADAM17+ in cell from five fields per sample in each group was quantified and reported as relative fluorescence units (RFUs). *p < 0.05 compared with control group, #p < 0.05 compared with conditioned medium-treated group, n = 3. B Representative confocal images showing the subcellular localization of ADAM17 and vWF in RAECs treated with stretch injured-conditioned medium, exogenous S100B, and injured medium plus ONO-2506 or FPS-ZM-1. Scale bar = 20 μm. The immunofluorescence intensity of vWF+/ADAM17+ in cells from five fields per sample in each group was quantified and reported as relative fluorescence units (RFUs). *p < 0.05 compared with control group, #p < 0.05 compared with conditioned medium-treated group, n = 3