Gli2 Antibody - #DF7541

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产品: Gli2 抗体
货号: DF7541
描述: Rabbit polyclonal antibody to Gli2
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P10070
RRID: AB_2841040

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Gli2 Antibody detects endogenous levels of total Gli2.
RRID:
AB_2841040
引用格式: Affinity Biosciences Cat# DF7541, RRID:AB_2841040.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CJS; Gli 2; GLI family zinc finger 2; GLI Kruppel family member GLI2; GLI2; GLI2_HUMAN; Glioma associated oncogene family zinc finger; HPE9; Oncogene GLI2; PHS2; Tax helper protein 1; Tax helper protein 2; Tax helper protein; Tax responsive element 2 holding protein; Tax responsive element 25 bp sequence binding protein; THP; THP1; THP2; Zinc finger protein GLI2;

抗原和靶标

免疫原:

A synthesized peptide derived from human Gli2, corresponding to a region within N-terminal amino acids.

基因/基因ID:
GLI2(2736)

研究领域

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

文献引用

1). Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex. Theranostics, 2023 (PubMed: 32042332) [IF=12.4]
2). Anoikis regulator GLI2 promotes NC cell immunity escape by TGF-β-mediated non-classic hedgehog signaling in colorectal cancer: based on artificial intelligence and big data analysis. Aging, 2023 (PubMed: 38159250) [IF=3.9]

Application: WB    Species: human    Sample:

Figure 9. GLI2 promoted drug tolerance. (A) Expression level of GLI2 after siRNA treatment in colorectal cancer cell (lovo) and gastric cancer by (a) PCR assay and (B) WB. (C) CCK-8 assay. Alive and dead cell staining in (D) colorectal cancer and (E) gastric cancer.
3). (‑)‑Epigallocatechin‑3‑gallate inhibits bladder cancer stem cells via suppression of sonic hedgehog pathway. ONCOLOGY REPORTS, 2019 (PubMed: 31180522) [IF=3.8]

Application: WB    Species: human    Sample: Bladder CSCs

Figure 3.| - EGCG induces apoptosis and reduces the expression of cell proliferation-related proteins on bladder CSCs. Bladder CSCs were treated with different concentrations of EGCG for 7 days. (D) The expression levels of Shh signaling pathway-related proteins including Shh, Smo, Gli1 and Gli2 were detected by western blot analysis. Data are expressed as mean ± standard deviation. *P<0.05, **P<0.01 vs. the EGCG-0 µm group. EGCG, (−)-epigallocatechin-3-gallate; CSCs, cancer stem cells; PCNA, proliferating cell nuclear antigen; Shh, sonic hedgehog protein; Smo, smoothened homolog; Gli, zinc finger protein GLI.
4). Effects of sulforaphane on prostate cancer stem cells-like properties: In vitro and molecular docking studies. Archives of biochemistry and biophysics, 2024 (PubMed: 39549984) [IF=3.8]
5). Targeting the RAS axis alleviates silicotic fibrosis and Ang II-induced myofibroblast differentiation via inhibition of the hedgehog signaling pathway. Toxicology Letters, 2019 (PubMed: 31181250) [IF=2.9]

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