Phospho-Nrf2 (Ser40) Antibody - #DF7519

Fig. 4. Effects of KAN0438757 on the regulation of Nrf2/HO-1 pathways. (A) The levels of HO-1, Nrf2, P-Nrf2, Keap 1, and NQO-1 proteins in mice with induced FAEE-SAP, determined by Western blot among DMSO + NS, 757 + NS, DMSO + FAEE-SAP, and K-757 + FAEE-SAP groups their quantification. (B) Western blot assessment of the protein levels of HO-1, Nrf2, P-Nrf2, Keap 1, and NQO-1 in RAW264.7 cells stimulated with LPS. (C) Quantification of the levels of the Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 proteins in mice with induced FAEE-SAP and (D) RAW264.7 cells stimulated with LPS by Western blot. Significance (exact p values as indicated) was determined by a one-way ANOVA test. Data represent three or more experiments and as mean value ± SEM: n = 5, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Fig. 6. The impact of ellagic acid treatment on arsenic-induced Nrf2/Keap1 signaling pathway in the hippocampus and HT22 cells. (A) Western blot for p-Nrf2, Nrf2 and Keap1 in the hippocampus. (B-D) Relative density analysis of the p-Nrf2, Nrf2 and Keap1 protein bands in the hippocampus. (E) Quantification of Keap1 mRNA levels in the hippocampus. (F) Western blot for Nucleus Nrf2, Nrf2 and Keap1 in HT22 cells. (G-I) Relative density analysis of the Nucleus Nrf2, Nrf2 and Keap1 protein bands in HT22 cells. (J) Quantification of Keap1 mRNA levels in HT22 cells. Data are presented as mean ± SD (n=3). * significant difference compared to the control group (*P

Fig. 7 Eriodictyol up-regulates VDR expression and activates the Nrf2/HO-1 signaling pathway. A VDR expression in the mouse brain was detected using immunohistochemical staining. Then, B the VDR protein expression level in the mouse cortex and hippocampus was measured using Western blotting. C The VDR protein expression level in HT-22 cells was measured using Western blotting, and D its relative mRNA expression level was detected using q-PCR. E The levels of Nrf2, p-Nrf2 and HO-1 in the mouse cortex and hippocampus were measured using Western blotting. F The levels of Nrf2, p-Nrf2 and HO-1 in HT-22 cells were measured using Western blotting. G The level of Nrf2 in the nucleus and cytoplasm of HT-22 cells was detected using Western blotting, and Lamin B1 and β-actin were used as loading controls for the nuclear and cytoplasmic proteins, respectively. The data are presented as the means ± SD of three experiments. **P < 0.01 and ***P < 0.001 compared with the Aβ1–42 oligomer group. ##P < 0.01 compared with the control group

Fig. 5. AS-IV modulates pSmad3C/3L and Nrf2/HO-1 pathways in mice treated with DCC for 12 weeks. (A), (B), (C), (F), (G), (H), (I), (M) Hepatic expression and distribution pattern of proteins (TGF-β1, pSmad3C, pSmad3L, pNrf2) by immunofluorescent staining, and relative fluorescent intensity of TGF-β1, pSmad3C, pSmad3L, pNrf2 in the liver (Magnification × 400); (D), (E), (K), (L) Representative immunoblots and relative protein levels of TGF-β1, pSmad3C, pSmad3L, pSmad2C, pSmad2L, PAI-1, pNrf-2, Nrf-2, HO-1, NQO1. (J), (N) The Nrf-2 expression was evaluated by immunohistochemistry (Magnification × 400), and quantitation (% of the positively stained area) of Nrf2 by immunohistochemistry; (O) the correlation analysis of pSmad3C and pNrf2; (P) the correlation analysis of pSmad3L and pNrf2. Data are presented as mean ± SD (n = 3 for each group). #P < 0.05 VS. Control. # #P < 0.01 VS. Control. *P < 0.05 VS. Model. **P < 0.01 VS. Model.