产品: TRPM7 抗体
货号: DF7513
描述: Rabbit polyclonal antibody to TRPM7
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q96QT4
RRID: AB_2841012

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 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TRPM7 Antibody detects endogenous levels of total TRPM7.
RRID:
AB_2841012
引用格式: Affinity Biosciences Cat# DF7513, RRID:AB_2841012.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; Transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRPM7; TRPM7_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human TRPM7, corresponding to a region within C-terminal amino acids.

基因/基因ID:

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

文献引用

1). Stem Leydig cells support macrophage immunological homeostasis through mitochondrial transfer in mice. Nature communications, 2024 (PubMed: 38459012) [IF=16.6]

Application: WB    Species: Mouse    Sample:

Fig. 5: TRPM7 is needed for mitochondrial transfer via TNT. A Heatmap showing the expression of pattern recognition receptors on BMSCs and SLCs. B Western blot analysis of TRPM7. C Quantitative analysis of TRPM7. Data are presented as the means ± SDs, n = 3 biological replicates for each group, one-way ANOVA. D Flow cytometry analysis of the mitochondrial transfer rate. E Quantitative analysis of the mitochondrial transfer rate. Data are presented as the means ± SDs, n = 3 biological replicates for each group, unpaired two-tailed Student’s t test. F The percentage of mitochondria transferred to macrophages treated with H2O2. G Quantitative analysis of the mitochondrial transfer rate. Data are presented as the means ± SDs. n = 3 biological replicates for each group, unpaired two-tailed Student’s t test. H The percentage of mitochondria transferred to macrophages after treatment with blebbistatin. I Quantitative analysis of the mitochondrial transfer rate. Data are presented as the means ± SDs, n = 3 biological replicates for each group, unpaired two-tailed Student’s t test. J Macrophages cocultured with SLCsMito-DsRed. Scale bar, 10 μm; large scale bar, 5 μm. K Knockdown of TRPM7 reduced mitochondrial transfer rate. L Quantitative analysis of the mitochondrial transfer rate in vivo. Data are presented as the means ± SDs. n = 3 biological replicates for each group, unpaired two-tailed t test. M Bright field diagram of testicular (scale bar, 2 mm) and H&E staining of testis samples (scale bar, 50 μm). N, O Quantification of the testis weight of the whole body and germ cell layers, n = 6 biological replicates for each group. Data are presented as the means ± SDs, one-way ANOVA. P Immunostaining of α-SMA (red) and PNA (green). Scale bar, 50 μm. Q Quantitative analysis of the PNA+ cells. n = 6 biological replicates for each group. Data are presented as the means ± SDs, one-way ANOVA. All box-and-whisker plots denote the maximum (top whisker), 75th (top edge of box), 25th (bottom edge of box), and minimum (bottom whisker) percentiles and the median (line in box). Source data are provided as a Source Data file.

2). Phosphorus magnesium fiber regulates macrophage polarization through TRPM7 to accelerate wound healing. Applied Materials Today, 2023 [IF=7.2]

Application: WB    Species: Mouse    Sample:

Fig. 4. PMF promoted wound healing through TRPM7-ERK1/2. (A) The release content and schematic diagram of magnesium ions in the wound site on the 3rd, 7th, and 15th days. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, n = 3 (B) Western blot analysis of the expression of TRPM7 and phosphorylated ERK1/2 proteins. (C) Quantitative analysis of related protein expression levels in the PMF treatment group compared with the control group. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. (D) Immunofluorescent staining images of skin tissue sections on day 7. CD68: pan-macrophage marker (red). TRPM7: (green). Scale bar: 40 μm. (E) Statistical summary of fluorescent expressing of TRPM7/CD68 expressing. ns: no significance

Application: IF/ICC    Species: Mouse    Sample:

Fig. 4. PMF promoted wound healing through TRPM7-ERK1/2. (A) The release content and schematic diagram of magnesium ions in the wound site on the 3rd, 7th, and 15th days. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, n = 3 (B) Western blot analysis of the expression of TRPM7 and phosphorylated ERK1/2 proteins. (C) Quantitative analysis of related protein expression levels in the PMF treatment group compared with the control group. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. (D) Immunofluorescent staining images of skin tissue sections on day 7. CD68: pan-macrophage marker (red). TRPM7: (green). Scale bar: 40 μm. (E) Statistical summary of fluorescent expressing of TRPM7/CD68 expressing. ns: no significance

3). Inhibition of the RIP3/MLKL/TRPM7 necroptotic pathway ameliorates diabetes mellitus-induced erectile dysfunction by reducing cell death, fibrosis, and inflammation. Frontiers in pharmacology, 2024 (PubMed: 39329120) [IF=5.6]

4). Gallium ions promote osteoinduction of human and mouse osteoblasts via the TRPM7/Akt signaling pathway. Molecular Medicine Reports, 2020 (PubMed: 32945378) [IF=3.4]

Application: WB    Species: human    Sample: MC3T3 cells

Figure 3. |Effect of TRPM7 siRNAs on TRPM7 protein expression in MC3T3‑E1 and hFOB1.19 cells. (A) Total and (B) membrane TRPM7 protein expression levels in MC3T3 cells following transfection with TRPM7 siRNAs.

5). Novel benzoylurea derivative decreases TRPM7 channel function and inhibits cancer cells migration. Channels (Austin, Tex.), 2024 (PubMed: 39212541) [IF=3.3]

Application: WB    Species: Human    Sample: MCF-7 cells

Figure 4. SUD inhibits TRPM7 expression through PI3K/Akt signaling pathway. (a) Effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in MCF-7 cells examined by qPCR. (b) effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in BGC-823 cells examined by qPCR. ***, **** denote significant difference with control group, p

6). Circ_0021155 can participate in the phenotypic transformation of human vascular smooth muscle cells via the miR-4459/TRPM7 axis. Biochemical and biophysical research communications, 2022 (PubMed: 36155059) [IF=2.5]

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