CCR2 Antibody - #DF7507

Figure 3 M1 macrophage infiltration into the ovaries is the main reason for the overactivation of primordial follicles in HFD mice. (A) qPCR analysis of the macrophage chemokine receptors in the ovaries of the ND and HFD mice. (B) Immunofluorescence detection of CCR2 (green) and F4/80 (red) in ND, HFD, and HFD+CVC mice (left panel), or in the control, LPS, and LPS+CVC treated mice (right panel). DNA was stained with DAPI (blue). Scale bar = 50 µm. (C) Representative H&E staining images and follicle number counts in the ovaries of HFD mice treated with CVC (n = 3), compared to the HFD mice (n = 5). ND mice were used as controls (n = 5). Scale bar = 50 μm. (D) Representative H&E staining images and follicle number counts in the ovaries of the control, LPS, and LPS+CVC treated mice (n = 5). Scale bar = 50 μm. Red arrows represent primordial follicles. Black arrows represent growing follicles.

FIGURE 3 | SACC cells derived CCL2 activated the expression of CCR2 in TAMs. SACC-83 cells and macrophages were co-cultured to simulate the interactions between SACC cells and TAMs. (A) The concentration of CCL2 in the conditioned media was examined by ELISA. The expression of CCL2 and CCR2 in the solely or co-cultured SACC-83 cells and macrophages were examined by qRT-PCR (B) and western blot (C).

FIGURE 3 | SACC cells derived CCL2 activated the expression of CCR2 in TAMs. SACC-83 cells and macrophages were co-cultured to simulate the interactions between SACC cells and TAMs. (A) The concentration of CCL2 in the conditioned media was examined by ELISA. The expression of CCL2 and CCR2 in the solely or co-cultured SACC-83 cells and macrophages were examined by qRT-PCR (B) and western blot (C).The knockdown effect of CCL2 in SACC-83 cells was measured by qRT-PCR (D), western blot (E), and immunofluorescence (F).

FIGURE 3 | SACC cells derived CCL2 activated the expression of CCR2 in TAMs. SACC-83 cells and macrophages were co-cultured to simulate the interactions between SACC cells and TAMs. (A) The concentration of CCL2 in the conditioned media was examined by ELISA. The expression of CCL2 and CCR2 in the solely or co-cultured SACC-83 cells and macrophages were examined by qRT-PCR (B) and western blot (C).The knockdown effect of CCL2 in SACC-83 cells was measured by qRT-PCR (D), western blot (E), and immunofluorescence (F).

Figure 2. |Expression of possible target chemokine receptors of CCL7 in primary NSCLC and bone metastasis tissues. A-C. IHC detection of CCR1, CCR2 and CCR3 expression in primary NSCLC and bone metastasis tissues. CCR1, CCR2 and CCR3 were mainly expressed on the cell membrane and in cytoplasm (arrow). D. qRT-PCR measurement of mRNA levels of CCR1, CCR2, CCR3 in primary NSCLC and bone metastasis tissues. *: P < 0.05.