Occludin Antibody - #DF7504

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产品: Occludin 抗体
货号: DF7504
描述: Rabbit polyclonal antibody to Occludin
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat, Pig
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: Q16625
RRID: AB_2841004

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Pig
克隆:
Polyclonal
特异性:
Occludin Antibody detects endogenous levels of total Occludin.
RRID:
AB_2841004
引用格式: Affinity Biosciences Cat# DF7504, RRID:AB_2841004.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Ocln; OCLN_HUMAN; Tight junction protein occludin;

抗原和靶标

免疫原:

A synthesized peptide derived from human Occludin, corresponding to a region within the internal amino acids.

基因/基因ID:
OCLN(100506658)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Dietary fiber alleviates alcoholic liver injury via Bacteroides acidifaciens and subsequent ammonia detoxification. Cell host & microbe, 2024 (PubMed: 38959900) [IF=20.6]
2). Gingerenone A Attenuates Ulcerative Colitis via Targeting IL-17RA to Inhibit Inflammation and Restore Intestinal Barrier Function. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38639442) [IF=15.1]

Application: IF/ICC    Species: Mouse    Sample:

Figure 2. GA suppressed inflammation and protected against intestinal barrier dysfunction to attenuate colitis in mice. A) Inflammatory cytokines (IL‐1β, IL‐6, IL‐17, and TNF‐α) levels in colonic tissues (n = 6). B) Inflammatory cytokines (IL‐1β, IL‐6, IL‐17, and TNF‐α) levels in serum (n = 6). C) MPO activity in colonic tissues (n = 6). D,E) NIMP‐R14 positive expression level, and representative IHC pictures of NIMP‐R14 protein (n = 6). F) Representative immunofluorescence images of ZO‐1, Occludin, and Muc‐2 in colonic tissues (n = 3). G) Serum FD4 level (n = 6) and the fluorescence intensities of ZO‐1, Occludin, and Muc‐2 (n = 3). H) Pearson correlation heatmap circos among pro‐inflammation cytokines levels, intestinal barrier function proteins, and pharmacodynamic evaluation index in different groups (n = 3). All data are expressed as mean ± S.E.M. *P < 0.05 and **P < 0.01 compared with the control group (Ctrl), #P < 0.05 and ## P < 0.01 compared with the DSS group.
3). The role of the microbiota–gut–brain axis in methamphetamine-induced neurotoxicity: Disruption of microbial composition and short-chain fatty acid metabolism. Acta Pharmaceutica Sinica B, 2024 (PubMed: 39664442) [IF=14.7]
4). Hepatic factor MANF drives hepatocytes reprogramming by detaining cytosolic CK19 in intrahepatic cholangiocarcinoma. Cell death and differentiation, 2024 (PubMed: 39972058) [IF=13.7]
5). Hepatic factor MANF drives hepatocytes reprogramming by detaining cytosolic CK19 in intrahepatic cholangiocarcinoma. Cell death and differentiation, 2024 (PubMed: 39972058) [IF=13.7]
6). Dietary palmitoleic acid reprograms gut microbiota and improves biological therapy against colitis. Gut microbes, 2023 (PubMed: 37203220) [IF=12.2]
7). Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway. Gut Microbes, 2021 (PubMed: 33769187) [IF=12.2]

Application: IHC    Species: mouse    Sample: colon

Figure 5. |FnEVs increase gut barrier leakage in experimental colitis models. (a) Representative images and ex vivo imaging with the intestine, liver, heart, spleen and kidney of mice. (b) Relative fluorescence intensity of translocated EGFP-labeled E.coli in every tissues. (c) Representative images of immunohistochemical stainings of ZO-1, claudin-1 and occludin in the colon on day 3 after colitis induction. Scale bar = 50 um.
8). Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice. Genomics, proteomics & bioinformatics, 2022 (PubMed: 35609771) [IF=11.5]
9). Water-extracted Lonicera japonica polysaccharide attenuates allergic rhinitis by regulating NLRP3-IL-17 signaling axis. Carbohydrate Polymers, 2022 (PubMed: 36184153) [IF=10.7]
10). Vitamin K1 ameliorates lipopolysaccharide-triggered skeletal muscle damage revealed by faecal bacteria transplantation. Journal of cachexia, sarcopenia and muscle, 2024 (PubMed: 38018317) [IF=9.4]

Application: WB    Species: Mouse    Sample:

Figure 2 Faecal bacteria transplantation from S‐res mice alleviates LPS‐triggered muscle damage and intestinal barrier disruption. (A) Description of the steps of FMT experiment. (B) The score for Severity of sepsis in Sen and Res. (C) H&E staining in the GA muscle. Representative histological images on day 9 postoperatively (400× magnification). Scale bar = 100 μm. Comparison of muscle fibre diameter and CSA of muscle fibre in mice from each group. (D) Western blot analysis of MuRF‐1 and MAFbx protein levels in TA tissue in Sen and Res groups. The results were normalized to the expression of GAPDH. (E) Western blot analysis of ZO‐1 and occludin protein levels in colon tissue in Sen and Res groups. The results were normalized to the expression of GAPDH. Data are means ± SEM. P values determined through Student's t test and ordinary one‐way ANOVA. Sen versus Res: # P < 0.05, ## P < 0.01, ### P < 0.001. Sen and Res versus Ctrl: **P < 0.01, ***P < 0.001. Sen versus ABX: $ P < 0.05.
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