NLRP3 Antibody - #DF7502

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产品: NLRP3 抗体
货号: DF7502
描述: Rabbit polyclonal antibody to NLRP3
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q96P20
RRID: AB_2841002

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
NLRP3 Antibody detects endogenous levels of total NLRP3.
RRID:
AB_2841002
引用格式: Affinity Biosciences Cat# DF7502, RRID:AB_2841002.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AGTAVPRL; AII/AVP; Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; LRR and PYD domains-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLR family pyrin domain containing 3; NLRP3; PYPAF 1; PYPAF1; PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human Cryopyrin, corresponding to a region within C-terminal amino acids.

基因/基因ID:
NLRP3(114548)

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Activation of NLRP3 in microglia exacerbates diesel exhaust particles-induced impairment in learning and memory in mice. ENVIRONMENT INTERNATIONAL, 2020 (PubMed: 31999974) [IF=10.3]

Application: IHC    Species: mouse    Sample: brain

Fig. 4. Nlrp3 knockout (KO) mice were resistant to diesel exhaust particles (DEPs)-induced neurotoxicity. D: Enhanced NLRP3 expression was observed in DEPs-treated WT mice, but not in Nlrp3−/− mice exposed to DEPs.
2). Lactobacillus rhamnosus GG ameliorates triptolide-induced liver injury through modulation of the bile acid-FXR axis. Pharmacological research, 2024 (PubMed: 38908615) [IF=9.1]
3). Dietary disodium fumarate supplementation alleviates subacute ruminal acidosis (SARA)-induced liver damage by inhibiting pyroptosis via mitophagy-NLRP3 inflammasome pathway in lactating Hu sheep. Frontiers in Immunology, 2023 (PubMed: 37275885) [IF=5.7]

Application: WB    Species: Hu Sheep    Sample: liver tissue

Figure 5 Effect of DF supplementation on the activation of NLRP3 inflammasome. The expression of NLRP3 inflammasome-related (A) genes and (B–D) proteins in the liver tissue of Hu sheep were detected. n=6 per group and the values are the mean ± SEM. *P
4). Prebiotics alleviate cartilage degradation and inflammation in post-traumatic osteoarthritic mice by modulating the gut barrier and fecal metabolomics. Food & function, 2023 (PubMed: 37077156) [IF=5.1]
5). Shionone alleviates NLRP3 inflammasome mediated pyroptosis in interstitial cystitis injury. International Immunopharmacology, 2021 (PubMed: 33223465) [IF=4.8]
6). Phlorizin ameliorates myocardial fibrosis by inhibiting pyroptosis through restraining HK1-mediated NLRP3 inflammasome activation. Heliyon, 2023 (PubMed: 38027628) [IF=4.0]

Application: WB    Species: Mouse    Sample:

Fig. 10 The effects of PHL on expression of the signal pathway proteins HK1 (B), NLRP3 (C), ASC (D), Caspase-1 (E), and GSDMD-N (G) in ISO-induced mice (A and F). Data are shown as the mean ± SEM (n = 3). ##p < 0.01 vs. CONT group; **p < 0.01, *p < 0.05 vs. ISO group.
7). ZBP1 promotes hepatocyte pyroptosis in acute liver injury by regulating the PGAM5/ROS pathway. Annals of hepatology, 2024 (PubMed: 38331384) [IF=3.7]
8). Subacute ruminal acidosis induces pyroptosis via the mitophagy-mediated NLRP3 inflammasome activation in the livers of dairy cows fed a high-grain diet. Journal of dairy science, 2024 (PubMed: 38278294) [IF=3.7]
9). PHLDA1 Blockade Alleviates Cerebral Ischemia/Reperfusion Injury by Affecting Microglial M1/M2 Polarization and NLRP3 Inflammasome Activation. Neuroscience, 2022 (PubMed: 35093445) [IF=2.9]
10). Malus toringoides (Rehd.) Hughes decoction alleviates ISO-induced cardiac fibrosis by inhibiting cardiomyocyte inflammation and pyroptosis via the HK1/NLRP3-signaling pathway. Bioscience, biotechnology, and biochemistry, 2024 (PubMed: 38697933) [IF=1.4]

Application: WB    Species: Mouse    Sample:

Figure 9.Effect of Ese on the expression of pathway proteins and mechanism. (a) Effect of Ese on the levels of signal pathway proteins HK1 (b), NLRP3 (c), ASC (d), and caspase-1 (e) in ISO-induced mice. Data are presented as the mean ± SEM (n = 3).

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