Nephrin Antibody - #DF7501

FIGURE 5Podocyte injury and C3aR expression were evaluated in STZ-diabetic DKD rats. (A) Representative images of nephrin and WT1 expressed in glomeruli of DKD rats by IHC staining (n = 4). (B) Representative images of C3aR expressed in glomeruli of DKD rats by IHC staining (n = 4). (C and D) Quantitative analysis of nephrin, WT1 and C3aR was performed using Image-Pro Plus 6.0 software (n = 4, more than 20 glomeruli). (E and F) The colocalization of nephrin and C3aR in human renal biopsies and DKD kidney tissues by a commercial double staining kit. 400× original magnification; scale bar, 50 µm. Data are presented as the means ± SEM, and p < .05 was considered to be statistically significant

Figure 2 ADAM10 knockdown inhibits HG-induced podocyte apoptosis. (A) Podocyte markers were evaluated using western blot analysis. (B) Podocyte apoptosis was evaluated using the TUNEL assay. (C) Apoptotic rate was obtained according to the results of the TUNEL assay. (D) Expression levels of proteins related to apoptosis were assessed using western blot analysis. ***P

Figure 3 (a) Effect of Keluoxin and Irbesartan on LC3, p62, and nephrin proteins in renal tissues of DN-induced rats. (a) The levels of LC3, p62, and nephrin proteins from renal tissues were detected by western blot and normalized to β-actin. (b) The expression of LC3-II. (c) The ratio of LC3-ll/LC3-l. (d) The expression of p62. (e) The expression of nephrin protein. (f) Mean fluorescence intensity of nephrin was measured for each group.  ∗P < 0.05 and  ∗∗P < 0.01 versus the normal group;  #P < 0.05 and  ##P < 0.01 versus the model group.