Phospho-beta Catenin (Ser33/Ser37/Thr41) Antibody - #DF2989

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产品: 磷酸化 beta Catenin (Ser33/Ser37/Thr41) 抗体
货号: DF2989
描述: Rabbit polyclonal antibody to Phospho-beta Catenin (Ser33/Ser37/Thr41)
应用: WB IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
蛋白号: P35222
RRID: AB_2840968

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-beta Catenin (Ser33/Ser37/Thr41) Antibody detects endogenous levels of beta Catenin only when phosphorylated at Ser33/37/Thr41.
RRID:
AB_2840968
引用格式: Affinity Biosciences Cat# DF2989, RRID:AB_2840968.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Beta catenin; Beta-catenin; Cadherin associated protein; Catenin (cadherin associated protein), beta 1, 88kDa; Catenin beta 1; Catenin beta-1; CATNB; CHBCAT; CTNB1_HUMAN; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923; OTTHUMP00000162082; OTTHUMP00000165222; OTTHUMP00000165223; OTTHUMP00000209288; OTTHUMP00000209289;

抗原和靶标

免疫原:

A synthesized peptide derived from human β-Catenin around the phosphorylation site of Ser33/37/Thr41.

基因/基因ID:
CTNNB1(1499)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Exercise-induced Musclin determines the fate of fibro-adipogenic progenitors to control muscle homeostasis. Cell stem cell, 2024 (PubMed: 38232727) [IF=19.8]
2). MANF serves as a novel hepatocyte factor to promote liver regeneration after 2/3 partial hepatectomy via doubly targeting Wnt/β-catenin signaling. Cell death & disease, 2024 (PubMed: 39289348) [IF=8.1]
3). Ursolic Acid promotes apoptosis and mediates transcriptional suppression of CT45A2 gene expression in NSCLCs harboring EGFR T790M. British Journal of Pharmacology, 2019 (PubMed: 31322286) [IF=6.8]

Application: WB    Species: mouse    Sample: tumour

FIGURE 7 | Ursolic acid (UA) inhibited CT45A2 mRNA expression and β-catenin/TCF4 signalling pathway in vivo. (A) Left: UA inhibited CT45A2 mRNA expression in tumour tissues. Tumour tissues from groups treated with vehicle, UA (25 mg kg-1) or erlotinib (20 mg kg-1), were harvested for total RNA extraction and mRNA levels were measured with PCR. GAPDH is used as a control. Right: CT45A2 mRNA levels in Fig. 7A, as analyzed by Image J software. (B) Left: UA inhibited β-catenin/TCF4 signalling protein expression in tumour tissues. Tumour tissues from groups treated with vehicle, UA (25 mg kg-1) or erlotinib (20 mg kg-1), were immunoblotted with antibodies to detect β-catenin, GSK-3β, p-βcatenin(Ser33/37/Thr41), p-GSK-3β(Ser9), TCF4 and GAPDH. Right: Analysis of western blotting results in Fig. 7B. Protein levels were quantified using gray value analyses by Image J software.
4). 20(S)- Protopanaxadiol suppresses hepatic stellate cell activation via WIF1 demethylation-mediated inactivation of the Wnt/β-catenin pathway. Journal of Ginseng Research, 2023 (PubMed: 37397420) [IF=6.8]
5). Betulinic acid Attenuated Bleomycin-induced Pulmonary Fibrosis by Effectively Intervening Wnt/β-catenin Signaling. PHYTOMEDICINE, 2021 (PubMed: 33341025) [IF=6.7]

Application: WB    Species: Mice    Sample: Mlg and PPF cells

Fig. 4. BA inhibited Wnt3a-induced nuclear translocation of β-catenin. BA (5, 10 and 20 μМ) was added to Mlg and PPF cells for 2 h, then cells were dealt with Wnt3a (100ng/ml) for an additional 2 h. (A-B) We followed the manufacturers’ instructions to fractioned of (A) Mlg and (B) PPF cells, and analyzed the specimen with western blot. GAPDH and Lamin B are respectively used as internal reference for cytoplasm and nucleus. As shown below the blots, we used densitometry to quantified the results of immunoblot. (C-D) β-catenin localization in (C) Mlg and (D) PPF cells were detected by immunofluorescence and the pictures were dealt with photoshop (Scale bar, 60 µm). Results are shown as mean ± SEM, n = 3.

Application: IF/ICC    Species: Mice    Sample: Mlg and PPF cells

Fig. 4. BA inhibited Wnt3a-induced nuclear translocation of β-catenin. BA (5, 10 and 20 μМ) was added to Mlg and PPF cells for 2 h, then cells were dealt with Wnt3a (100ng/ml) for an additional 2 h. (A-B) We followed the manufacturers’ instructions to fractioned of (A) Mlg and (B) PPF cells, and analyzed the specimen with western blot. GAPDH and Lamin B are respectively used as internal reference for cytoplasm and nucleus. As shown below the blots, we used densitometry to quantified the results of immunoblot. (C-D) β-catenin localization in (C) Mlg and (D) PPF cells were detected by immunofluorescence and the pictures were dealt with photoshop (Scale bar, 60 µm). Results are shown as mean ± SEM, n = 3.
6). Microcystin-LR accelerates follicular atresia in mice via JNK-mediated adherent junction damage of ovarian granulosa cells. Ecotoxicology and Environmental Safety, 2023 (PubMed: 36731181) [IF=6.2]
7). Chelerythrine chloride inhibits the progression of colorectal cancer by targeting cancer-associated fibroblasts through intervention with WNT10B/β-catenin and TGFβ2/Smad2/3 axis. Phytotherapy research : PTR, 2023 (PubMed: 37402476) [IF=6.1]
8). Fam70A Binds Wnt5a to Regulate Meiosis and Quality of Mouse Oocytes. Cell Proliferation, 2020 (PubMed: 32391621) [IF=5.9]

Application: WB    Species: mice    Sample: Oocytes

FIGURE 6 Fam70A and Wnt5a regulate β-catenin activity. A, CoIP showed that Fam70A interacted with β-catenin within oocytes. B, Co-IP showed that Wnt5a interacted with β-catenin within oocytes. C, Western blot showed that Fam70A depletion remarkably increased p-β- catenin level. D, Quantification of (C). E, Immunofluorescence showed that Fam70A depletion remarkably increased p-β-catenin at spindle poles. F, Western blot showed that Wnt5a knockdown remarkably increased p-β-catenin level. G, Quantification of (F). H, Western blot showed that APC knockdown remarkably decreased p-β-catenin level. I, Quantification of (H). Scale bar, 5 µm. *P < .05

Application: IF/ICC    Species: mice    Sample: Oocytes

FIGURE 6 Fam70A and Wnt5a regulate β-catenin activity. A, CoIP showed that Fam70A interacted with β-catenin within oocytes. B, Co-IP showed that Wnt5a interacted with β-catenin within oocytes. C, Western blot showed that Fam70A depletion remarkably increased p-β- catenin level. D, Quantification of (C). E, Immunofluorescence showed that Fam70A depletion remarkably increased p-β-catenin at spindle poles. F, Western blot showed that Wnt5a knockdown remarkably increased p-β-catenin level. G, Quantification of (F). H, Western blot showed that APC knockdown remarkably decreased p-β-catenin level. I, Quantification of (H). Scale bar, 5 µm. *P < .05
9). Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway. International Journal of Molecular Medicine, 2018 (PubMed: 30272329) [IF=5.7]
10). The Effect of Cholesterol Efflux on Endothelial Dysfunction Caused by Oxidative Stress. International journal of molecular sciences, 2023 (PubMed: 36983012) [IF=5.6]

Application: WB    Species: human    Sample:

Figure 5. LXR-623 can inhibit ER stress and the Wnt/β-catenin pathway by promoting cholesterol efflux, and adding cholesterol extracellularly can induce ER stress and activate the Wnt/β-catenin pathway by increasing the deposition of intracellular cholesterol. (A–D) The expressions of GRP78, CHOP, β-catenin, and p-β-catenin were detected by Western blot. n = 3, ** p < 0.001 vs. the C group; ## p < 0.001 vs. the H2O2 + LXR-623 group. The C (control) group of LXR-623 and cholesterol were treated by DMSO and chloroform, respectively; H2O2, cells treated with H2O2 (500 μmol/L) for 24 h; LXR-623, cells treated with LXR-623 (5 μmol/L) for 24 h; H2O2 + LXR-623, cells treated with LXR-623 (5 μmol/L) for 2 h and then treated with H2O2 (500 µmol/L) for 22 h; CHOL, cells were treated by cholesterol (100 μmol/L) for 24 h; H2O2 + CHOL, cells were firstly treated by cholesterol (100 μmol/L) for 2 h, and then H2O2 (500 µmol/L) was used to treat cells for 22 h.
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